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Zhongjian Baotai

Beijing Biotai Co., Ltd. and all staff are dedicate-d to providing customers with the best techni-cal solutions, quality products and after-sales services, and strive to promote the developme-nt of China's food safety industry.

TEL：400-1888-518

Veterinary drug residue Tests

ELISA Kit for Evergreen Furazolidone (AOZ)

Nitrofuran residues in food pose a serious threat to public health. Nitrofurans are broad-spectrum antibiotics (including nitrofuran, furazolidone, furantoin and furacilin), which have very good antibacterial and pharmacokinetic properties, and have been widely used as growth-promoting additives for poultry, aquaculture and pigs. However, long-term experimental studies have found that nitrofuran drugs and metabolites can cause carcinogenesis and gene mutations in experimental animals. At present, the use of such drugs in animal treatment and feed is prohibited in the United States, Canada and the European Union, and all imported foods containing nitrofurans are prohibited. Therefore, to ensure the health of consumers, antibiotic residues in water sources and food products (such as meat) should be monitored. Detection of nitrofurans is inherently challenging because nitrofurans are rapidly metabolized after ingestion. However, the protein-bound metabolites formed can persist for a long time. AOZ is the main metabolite of furazolidone, which cannot be removed by usual cooking treatments and will be released from tissues under weak acid conditions, so furazolidone metabolites in edible tissues can be monitored and detected. The Biorex Furazolidone (AOZ) test kit uses the principle of enzyme-linked immunosorbent assay to detect the furazolidone metabolite AOZ in a sample. Detection principle The kit uses enzyme-linked immunosorbent assay to detect furazolidone. The micropores on the ELISA plate provided with the kit were pre-coated with AOZ antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. The AOZ in the standard and sample simultaneously competes with the enzyme label for the limited binding sites on the coated antibody, and the reaction is carried out for 30 minutes. The microwells are washed and the substrate reagent is added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the AOZ content in the standard/sample, a standard curve is constructed, and the AOZ concentration in the sample is calculated. The kit consists of Chinese name, English name, specification, AOZ microplate Microtitreplate12 × 8 wells, coated with AOZ antibody standard Standards6 × 1.5ml, and directly Furazolidone-HRPConjugateConc with enzyme marker concentrate. 0.2mL enzyme-labeled conjugate diluent ConjugateDiluent12ml, red solution washing solution/sample diluent concentrate WashSolution/SampleDiluent (20x) Concentrate,50ml,20X concentrated AOZ high-standard AOZspikingsolution1ml,50ng/ml substrate solution SubstrateSolution14mL stop solution StopSolution8mL microplate lid PlateCover

Evergreen Furaltanone (AMOZ) ELISA Kit

Nitrofuran residues in food pose a serious threat to public health. Nitrofurans are broad-spectrum antibiotics (including nitrofuran, furazolidone, furantoin and furacilin), which have very good antibacterial and pharmacokinetic properties, and have been widely used as growth-promoting additives for poultry, aquaculture and pigs. However, long-term experimental studies have found that nitrofuran drugs and metabolites can cause carcinogenesis and gene mutations in experimental animals. At present, the use of such drugs in animal treatment and feed is prohibited in the United States, Canada and the European Union, and all imported foods containing nitrofurans are prohibited. Therefore, to ensure the health of consumers, antibiotic residues in water sources and food products (such as meat) should be monitored. Detection of nitrofurans is inherently challenging because nitrofurans are rapidly metabolized after ingestion. However, the protein-bound metabolites formed can persist for a long time. AMOZ is the main metabolite of furalitones, which cannot be removed by usual cooking treatments and will be released from tissues under weak acid conditions. Therefore, furalitones metabolites in edible tissues can be monitored and detected. The Biorex Furaltanone (AMOZ) test kit uses the enzyme-linked immunosorbent principle to detect the furaltanone metabolite AMOZ in samples. Detection principle The kit uses enzyme-linked immunosorbent assay to detect furaldone. The microwells on the ELISA plate provided with the kit were pre-coated with AMOZ antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. AMOZ in the standard and sample simultaneously competes with the enzyme label for the limited binding sites on the coated antibody, and the reaction is carried out for 30 minutes. The microwells are washed and the substrate reagent is added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the AMOZ content in the standard/sample, a standard curve is constructed, and the AMOZ concentration in the sample is calculated. The kit consists of Chinese name, English name, specification, AMOZ microplate Microtitreplate12 × 8 wells, coated with AMOZ antibody standard Standards6 × 1.5ml, and directly Furaltadone-HRPConjugateConc with enzyme marker concentrate. 0.2mL enzyme-labeled conjugate diluent ConjugateDiluent12ml, red solution washing solution/sample diluent concentrate WashSolution/SampleDiluent (20x) Concentrate,50ml,20X concentrated AMOZ high-standard AMOZspikingsolution1ml,50ng/ml substrate solution SubstrateSolution14mL stop solution StopSolution8mL

Evergreen Sulfonamide Total Test Kit

The total amount of Evergreen sulfonamides detection kit used immunological principle to detect the residues of sulfonamides in honey in vitro. Sulfonamides are a class of organic synthetic compounds that are effective in treating bacterial and protozoal infections. Because these drugs can cause resistance in the body's immune system, they need to be monitored. Many countries have established maximum residue limits for sulfonamides, and the European Union, Jian'an University and the United States stipulate that the total amount of sulfonamides in edible tissues cannot exceed 100ppb. Detection principle The kit uses enzyme-linked immunosorbent assay to detect sulfonamide. The antigen is coated in a microplate, and the standard/sample and the antibody are added to the microplate sequentially. During incubation, the sulfonamide and the enzyme-labeled conjugate in the sample compete for binding to the sulfonamide antibody. After incubation, unbound enzyme-labeled conjugate and remaining antibody were removed by washing. The substrate reagent is then added, and the enzyme label reacts with the substrate. After incubation, stop solution was added to stop the reaction and the solution turned yellow. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. Kit composition reagent number Chinese name English name specification FT08 standard STANDARD ready-to-use type, 1ml/bottle FT08-008 microplate MICROTITERPLATE12 × 8 wells, coated antibody antigen FT08-009 washing buffer (50 ×)WASHBUFFER1 × 20ml, if the whole bottle is used, 20ml concentrate 980ml double deionized water, mix well. After dilution, it can be stored at 2-8 ℃ for 30 days. FT08-010 stop solution STOPSOLUTION1 mL × 8mL, colorless liquid, transparent bottle, red screw cap, dilute hydrochloric acid solution, ready-to-use FT08-011 substrate solution SUBSTRATESOLUTION1 mL × 14ml, transparent solution, can directly use FT08-012 enzyme marker CONJUGATE1 mL × 0.2mL, amber bottle FT08-014 sample diluent SAMPLEDILUENT1 mL × 30mL,20 × concentration, if dilute the whole bottle, 570ml double deionized water should be added and used on the same day. Microplate Cover PLATECOVER Incubation Cover Microplate Instructions IFU

Evergreen Tetracycline ELISA Kit

Evergreen Tetracycline ELISA Kit is suitable for in vitro quantitative detection of tetracycline in honey and muscle tissue. Tetracycline has antibacterial effect on various gram positive and gram negative bacteria, and it is a kind of broad spectrum antibiotic. Tetracyclines can persist in high concentrations in soil for long periods of time without exhibiting antimicrobial activity. The main effect of tetracyclines on human health is to increase the resistance of human pathogens. In addition, other health effects such as developmental toxicity are also associated with exposure to antibiotics. Detection principle The microwells on the microwell plate provided by the kit are pre-coated with tetracycline antibody. Tetracycline standard/sample and receptor were added to the microplate and incubated. The unbound reagent in the microplate is washed, the enzyme-labeled conjugate is added, and incubated again. The enzyme-labeled conjugate reacts with the receptor bound to the microplate. The microplate was washed again, the substrate reagent was added, and the substrate and the enzyme-labeled conjugate had a blue color reaction. The depth of color is inversely proportional to the amount of tetracycline in the standard/sample. After a period of time, add the stop solution and read the absorbance value using a microplate reader. A standard curve was constructed and the concentration of tetracycline in the sample was calculated. Kit composition 1) microplate: coated tetracycline antibody (vacuum packaged in aluminum foil bag, containing desiccant) 2) tetracycline standard 3) receptor: 3 bottles, freeze-dried powder, 2mL/bottle 4) receptor diluent: 10ml1 bottle 5) concentrated sample dilution buffer (10X):25ml2 bottles, which must be diluted before use 6) concentrated enzyme labeled conjugate: 100 μl,1 bottle 7) enzyme conjugate labeled diluent: 14ml,1 bottle 8) concentrated washing buffer (50X):20ml 9) substrate solution (TMB):14ml 10) stop solution: 6ml 11) microplate cover

Evergreen Streptomycin Assay Kit

The Evergreen streptomycin test kit uses immunological principles to detect streptomycin and dihydrostreptomycin in honey in vitro. Streptomycin is an aminoglycoside antibiotic produced by Streptomyces sp. Streptomycin and dihydrostreptomycin are similar in structure, pharmacokinetics and biological activity. Both drugs are used to treat bacterial infections in cattle, pigs, sheep and poultry. The European Union prohibits the use of streptomycin in beekeeping, while the United States allows its use in the treatment of piglet disease (which requires a four-week holiday). Antibiotics are not allowed to be detected in honey, so it is necessary to monitor streptomycin in honey. Antibiotics in honey can cause allergic reactions and increase drug resistance in humans. Detection principle The kit uses enzyme-linked immunosorbent assay to detect streptomycin. Streptomycin in honey was extracted with dilute wash buffer. The standard or sample extract and the enzyme-labeled conjugate are first added to the microwell, and then the antibody solution is added to start the reaction. During incubation, streptomycin and the enzyme-labeled conjugate in the sample compete for binding to the streptomycin antibody. After incubation, unbound enzyme-labeled conjugate and remaining antibody were removed by washing. The substrate reagent is then added, and the enzyme label reacts with the substrate. After incubation, stop solution was added to stop the reaction and the solution turned yellow. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. Kit composition reagent number Chinese name English name specification FB02 standard STANDARD ready-to-use type, 2ml/bottle FB02-08 microplate Microtitreplate12 × 8 wells, coated antibody binding protein FB02-09 washing buffer (50x) WashBuffer1 × 20ml, if the whole bottle is used, 20ml concentrate 980ml double deionized water, mix well. FB02-10 stop solution StopSolution1 mL × 14mL, colorless liquid, transparent bottle, red screw cap, dilute sulfuric acid solution, ready-to-use FB02-11 substrate solution SubstrateSolution1 mL × 14mL, transparent solution, can directly use FB02-12 enzyme marker Conjugate1 mL × 8mL, ready-to-use FB02-13 antibody solution ANTIBODYSOLUTION1 mL × 8mL, streptomycin antibody (monoclonal), ready-to-use microplate cap PLATECOVER cover microplate instruction IFU

Evergreen Quinolone Test Kit

Quinolones are indicated for the treatment of community-acquired and nosocomial infections and are bactericidal. It is widely distributed throughout the body with higher tissue permeability than concentrations in plasma, feces, bile, prostate tissue and lung tissue. When the drug is metabolized by the kidneys, the drug penetrates the urine and kidneys. Quinolones have an antibacterial effect on many bacteria, especially gram-negative bacteria. Most side effects are gastrointestinal reactions (nausea, dyspepsia, and vomiting) and central nervous system reactions, such as dizziness, insomnia, and headache. The Biorex Quinolone Assay Kit uses the enzyme-linked immunosorbent principle to detect quinolones (fluoroquinolones) in a sample. Detection principle The kit uses enzyme-linked immunosorbent assay to detect quinolones. The microwells on the ELISA plate provided with the kit were pre-coated with quinolone antibody. A specific enzyme-linked reaction is performed by first adding the standard/sample and the enzyme label to the microwell. During incubation, the quinolones in the standard and sample compete with the enzyme label simultaneously for the limited binding sites on the coated antibody. The microwells are washed and the substrate reagent is added. Enzyme labeling and substrate blue color reaction, after incubation, adding stop solution to stop the reaction, the solution turned yellow. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the quinolone content in the standard/sample, a standard curve is constructed, and the quinolone concentration in the sample is calculated. The kit consists of Chinese name, English name, specification, quinolone microplate Microtitreplate12 × 8 wells, coated with quinolone antibody standard Standards6 × 1.5ml, directly EnzymeConjugate amber bottle with enzyme marker concentrate, 100 μL/bottle high standard SpikingSolution1 × 1mL,100ng/mL washing concentrate WashSolution(50 ×)Concentrate 20mL ,50 × concentrated substrate solution SubstrateSolution amber plastic bottle, 1 × 14mL sample dilution concentrate SampleDiluent10X plastic bottle, blue lid, 2 × 25mL stop liquid StopSolution plastic bottle, red lid, 1 × 9mL microporous lid Platecover

Evergreen Chloramphenicol Test Kit-Highly Sensitive

Chloramphenicol is associated with aplastic anemia in humans, can affect reproduction in animals, and is hepatotoxic. Chloramphenicol is a broad-spectrum antibiotic widely used in livestock and poultry breeding, which can kill the main gram-negative bacteria in animals. Many countries prohibit the use of chloramphenicol in food animals. Therefore, it is necessary to monitor chloramphenicol in foods such as meat, milk, honey and aquatic products. Biorex Chloramphenicol Detection Kit-highly sensitive detection of chloramphenicol in honey, muscle, eggs and seafood/shrimp using the principle of enzyme-linked immunosorbent assay. Detection principle The kit uses enzyme-linked immunosorbent assay to detect chloramphenicol. The micropores on the enzyme label plate provided by the kit are pre-coated with secondary antibodies. Standards or samples, enzyme labels and antibodies are added to the microwells. During incubation, the chloramphenicol in the standard/sample simultaneously competes with the enzyme label for the binding site on the antibody. Unbound enzyme-labeled conjugate is removed by washing. Then the substrate reagent is added, and the enzyme label reacts with the substrate. After incubation, the stop solution is added to terminate the reaction. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. The kit consists of Chinese name, English name, specification, microplate Microtitreplate12 × 8 wells, coated with secondary chloramphenicol standard Standards6 bottle, 1.5ml/bottle enzyme marker EnzymeConjugate 250μL/bottle, amber bottle enzyme marker diluent EnzymeConjugateDiluent1 × 12ml, red solution, plastic bottle antibody solution AntibodySolution1 × 12ml, blue solution, plastic bottle washing buffer (50 ×)WashSolution(50X)Concentrate1 × 20ml termination solution StopSolution1 × 9mL, red cap plastic bottle substrate solution SubstrateSolution1 ml × 24ml, amber plastic bottle sample diluent (5X)SampleDiluent5X2 ml × 25ml, blue cap plastic bottle high standard solution SpikingSolution1 ml × 1ml,100ng/ml microplate cap PLATECOVER cover microplate when incubated

Evergreen Chloramphenicol Rapid Test Kit

Chloramphenicol is a broad-spectrum antibiotic widely used in livestock and poultry breeding, which can kill the main gram-negative bacteria in animals. The European Union prohibits the use of chloramphenicol in food animals. Antibiotic residues in food, such as chloramphenicol, can cause aplastic anemia, which poses a serious threat to public health. Therefore, many countries prohibit the use of chloramphenicol in food animals. Therefore, it is necessary to monitor chloramphenicol in foods such as meat, milk, honey and aquatic products. Evergreen chloramphenicol rapid test kit uses enzyme-linked immunosorbent principle to detect chloramphenicol and chloramphenicol glucuronide in urine and fish and shrimp. Detection principle The kit uses enzyme-linked immunosorbent assay to detect chloramphenicol. The microwells on the ELISA plate provided with the kit were pre-coated with antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. During incubation, both the standard and the sample compete with the enzyme label for the limited binding sites on the coated antibody. Unbound enzyme-labeled conjugate is removed by washing. The substrate reagent is then added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. Kit composition reagent number Chinese name English name specification FB03 standard STANDARD ready-to-use type, 1ml/bottle FB03-008 microplate Microtitreplate12 × 8 wells, coated antibody FB03-009 washing buffer (50 ×)WashBuffer1 × 20ml, if the whole bottle is used, 20ml concentrate 980ml double deionized water, mix well. After dilution, it can be stored at 2-8 ℃ for 30 days. FB03-010 stop solution StopSolution1 mL × 11mL, colorless liquid, transparent bottle, red screw cap, dilute hydrochloric acid solution, ready-to-use FB03-011 substrate solution SubstrateSolution1 mL × 13mL, transparent solution, can be directly used FB03-012 enzyme marker Conjugate1 mL × 11mL, amber bottle, ready-to-use microplate cap PLATECOVER cover microplate instructions IFU

Clenbuterol Evergreen rapid test kit

Clenbuterol is often used as a growth promoter in cattle and swine farming. When animals eat feed, they convert excess energy into fat, but when animals eat clenbuterol, the excess energy is converted into muscle, not fat. Clenbuterol is a bronchodilator that opens the airways by relaxing the tense muscles around the airways when asthma or chronic obstructive pulmonary disease strikes. Clenbuterol can cause anxiety, tremors, palpitations or tachycardia, and hypokalemia. The Evergreen clenbuterol rapid test kit uses the principle of enzyme-linked immunosorbent assay to detect clenbuterol in urine, serum and tissue samples. l sample: urine, serum and tissue l sample preparation: urine, serum-centrifugal tissue, including chicken, pork, turkey, beef-methanol extraction, evaporation and drying, recovery l analysis time: 45 minutes detection principle kit uses enzyme-linked immunosorbent assay to detect clenbuterol. The microwells on the ELISA plate provided with the kit were pre-coated with antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. During incubation, both the standard and the sample compete with the enzyme label for the limited binding sites on the coated antibody. Unbound enzyme-labeled conjugate is removed by washing. The substrate reagent is then added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. Kit composition specification composition/description 6 bottles, 1ml/bottle can be directly used 1 × 96-well microplate, 12 × 8 wells, coated antibody 1 × 20ml before use need to dilute concentrated washing buffer 50X concentration, if dilute the whole bottle (20mL), 980mL double deionized water should be added and mixed evenly. Once diluted, it can be stored at 2-8°C for 30 days. 1 x 11ml stop liquid colorless liquid, transparent bottle, red cap. Dilute hydrochloric acid solution, can be used directly. 1 x 13ml substrate solution clear solution, amber bottle, can be used directly. 1 × 25ml sample diluent transparent liquid, blue lid, can be directly used 1 × 11ml enzyme labeling conjugate amber bottle, white lid, can be directly used 1 × microplate lid 1 × instructions

Evergreen Clenbuterol Test Kit

Clenbuterol is often used as a growth promoter in cattle and swine farming. When animals eat feed, they convert excess energy into fat, but when animals eat clenbuterol, the excess energy is converted into muscle, not fat. Clenbuterol is a bronchodilator that opens the airways by relaxing the tense muscles around the airways when asthma or chronic obstructive pulmonary disease strikes. Clenbuterol can cause anxiety, tremors, palpitations or tachycardia, and hypokalemia. The Evergreen clenbuterol detection kit uses the principle of enzyme-linked immunosorbent assay to detect clenbuterol in samples. Detection principle The kit uses enzyme-linked immunosorbent assay to detect clenbuterol. The microwells on the ELISA plate provided with the kit were pre-coated with antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. During incubation, both the standard and the sample compete with the enzyme label for the limited binding sites on the coated antibody. Unbound enzyme-labeled conjugate is removed by washing. The substrate reagent is then added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. The kit consists of Chinese name, English name, specification, microplate Microtitreplate12 × 8 wells, coated with antibody clenbuterol standard Clenbuterol Standards7 bottle, enzyme marker EnzymeConjugate12mL/bottle, amber bottle, red solution antibody solution Anti-β-agonistsantibody1 × 12m, plastic bottle, blue solution concentrated washing solution (50 ×)WashSolution(50 ×)Concentrate1 × 20ml,50X concentrated substrate reagent SubstrateSolution1 × 24mL termination solution StopSolution1 × 8mL

Evergreen Beta-Doping Test Kit

Beta-agonists are often used as growth promoters in cattle and swine farming. When animals eat feed, they convert excess energy into fat, but when animals eat beta-stimulants, the excess energy is converted into muscle, not fat. Beta-stimulant is a bronchodilator that opens the airways by relaxing the tense muscles around the airways when asthma or chronic obstructive pulmonary disease attacks. Beta-agonists can cause anxiety, tremors, palpitations or tachycardia, and hypokalemia. The Evergreen beta-stimulant detection kit uses the principle of enzyme-linked immunosorbent assay to detect beta-stimulants in samples. Detection principle The kit uses enzyme-linked immunosorbent assay to detect β-agonists. The microwells on the ELISA plate provided with the kit were pre-coated with antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. During incubation, both the standard and the sample compete with the enzyme label for the limited binding sites on the coated antibody. Unbound enzyme-labeled conjugate is removed by washing. The substrate reagent is then added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. The kit consists of Chinese name English name specification microplate Microtitreplate12 × 8 wells, coated with antibody clenbuterol standard Clenbuterol Standards6 bottle enzyme marker EnzymeConjugate8mL ml/bottle, amber bottle dilution buffer 1 × DiluentionBuffer2 × 25ml, plastic bottle washing solution concentrate (50 ×)WashSolution(50 ×)Concentrate1 × 20ml,50X concentrated substrate solution SubstrateSolution1 × 14mL, amber plastic bottle termination solution StopSolution1 × 8mL

Evergreen Ractopamine Test Kit

Ractopamine is a synthetic beta adrenoceptor agonist. When animals eat ractopamine, it increases animal growth rate and lean meat percentage. Beta agonists, such as ractopamine, cause an increase in the animal's heart rate. The Evergreen ractopamine detection kit uses the enzyme-linked immunosorbent principle to detect ractopamine in urine, tissue, feed and milk. Detection principle The microwells on the enzyme plate provided by the kit are pre-coated with antibodies. A standard/sample solution, an enzyme label are added to the microwells, and during incubation, ractopamine in the standard/sample simultaneously competes with the enzyme label for the limited binding sites on the coated antibody. Unbound enzyme-labeled conjugate is removed by washing. The substrate reagent is then added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. Kit composition Chinese name English name specification premixed microplate PremixingMicrotiter12 × 8 wells, uncoated microplate Microtitreplate12 × 8 wells, coated ractopamine antibody ractopamine standard Ractopamine Standards7 bottle enzyme marker Conjugate1 × 0.2mL/bottle, plastic bottle enzyme marker diluent Conjugatediluent1 × 30mL dilution buffer (10 ×)DilutionBuffer 10 × 2 × 25mL, plastic bottle substrate concentrate Chromogen1 × 3mL substrate buffer Chromogenbuffer1 × 24mL, glass bottle washing solution concentrate (50 ×)WashSolution(50 ×)Concentrate1 × 20ml,50X concentrated stop solution StopSolution1 × 8mL

Test for Tetracycline in Baotai Charm II

The CharmII Tetracycline Kit can detect tetracycline drugs in milk, honey, eggs, fish, grains, pork, serum, tissue, urine and water. The detection limit of milk test kit meets the limit requirements of China, the United States, the European Union or CODEX, and is verified by NCIMS in the United States. Tissue test kit in accordance with US and EU/CODEXMRL. The Bottega CharmII Tissue, Serum and Urine Kit can be used to test any animal. The CharmII test can also be used to detect tetracycline in cereals and honey. The Bottega CharmII test runs on a Bottega CharmII system and is designed for use by veterinarians, laboratories and regulatory staff. The machine can provide comprehensive analysis of drug residues on a variety of matrices and meets the requirements of China, EU MRL and US FDA limits. The Baotai CharmII test uses binding reagents with specific receptor sites. The receptor is added to the sample along with the tracer-labeled antibiotic, and the antibiotic in the sample competes with the tracer-labeled antibiotic for binding sites. The amount of tracer bound to the receptor site is determined and compared to a quality control point. The quality control point is the threshold between negative or positive samples. The greater the amount of tracer measured, the lower the concentration of antibiotic residue in the sample; the smaller the amount of tracer measured, the greater the concentration of antibiotic residue in the sample. Features: The detection limit meets the requirements of MRL of China, EU and FDA of the United States. The detection time is 10-15 minutes. The same platform is suitable for detecting various antibiotic residues in different samples. It is suitable for dairy farms, veterinary stations, laboratories, pastures and control personnel. It has passed NCIMS verification in the United States and is a national standard and commodity inspection industry standard.

Detection of Bethel Charm II Sulfonamides

The CharmII Sulfonamides Kit detects sulfonamides in milk, honey, cereals and tissues. The detection limit of milk test kit meets the limit requirements of China, the United States, the European Union or CODEX, and is verified by NCIMS in the United States. Tissue detection kits can be used to test muscle tissue of animals, including cattle, pigs, chickens, and salmon. The Bottega CharmII also detects sulfonamides in cereals (such as popcorn) and honey. The Bottega CharmII test runs on a Bottega CharmII system and is designed for use by veterinarians, laboratories and regulators. The machine can provide comprehensive analysis of drug residues on a variety of matrices and meets the requirements of China, EU MRL and US FDA limits. The Baotai CharmII test uses binding reagents with specific receptor sites. The receptor is added to the sample along with the tracer-labeled antibiotic, and the antibiotic in the sample competes with the tracer-labeled antibiotic for binding sites. The amount of tracer bound to the receptor site is determined and compared to a quality control point. The quality control point is the threshold between negative or positive samples. The greater the amount of tracer measured, the lower the concentration of antibiotic residue in the sample; the smaller the amount of tracer measured, the greater the concentration of antibiotic residue in the sample. Features: The detection limit meets the requirements of MRL of China, EU and FDA of the United States. The detection time is 10-15 minutes. The same platform is suitable for detecting various antibiotic residues in different samples. It is suitable for dairy farms, veterinary stations, laboratories, pastures and control personnel. It is verified by FDA of the United States and is a national standard and a commodity inspection industry standard.

Detection of Baotai Charm II Macrolide

The CharmII Macrolides Kit detects macrolides in milk, honey, eggs, cereals, tissues, urine and water. The milk detection kit can detect macrolides and lincomamides in raw milk and pasteurized milk, and the detection limit meets the limit requirements of China, the United States, the European Union or CODEX. Tissue detection kits can be used to test muscle tissue of animals, including cattle, swine, and poultry. Urine testing predicts the level of macrolide residues in animal muscle. The Bottega CharmII test runs on a Bottega CharmII system and is designed for use by veterinarians, laboratories and regulatory staff. The machine provides comprehensive analysis of drug residues on a variety of matrices and meets China, EU MRL and US FDA limit requirements for CharmII testing using binding reagents with specific receptor sites. The receptor is added to the sample along with the tracer-labeled antibiotic, and the antibiotic in the sample competes with the tracer-labeled antibiotic for binding sites. The amount of tracer bound to the receptor site is determined and compared to a quality control point. The quality control point is the threshold between negative or positive samples. The greater the amount of tracer measured, the lower the concentration of antibiotic residue in the sample; the smaller the amount of tracer measured, the greater the concentration of antibiotic residue in the sample. Features: The detection limit meets the requirements of MRL in China, EU and FDA in the United States. The detection time is 10-15 minutes. The same platform detects a variety of antibiotic residues in different samples. It is suitable for dairy farms, veterinary stations, laboratories, pastures and control personnel. It is the industry standard for commodity inspection.

Detection of beta-lactams by Botai Charm II

Our company can provide a variety of CharmII beta-lactam test kits for milk, tissue and other foods. The Baotai CharmII β-lactam detection kit has been verified by FDA and "A" Pasteurized Milk Regulations (PMO). The CharmII test runs on a CharmII system and is designed for use by veterinarians, laboratories and regulatory staff. The machine can provide comprehensive analysis of drug residues on a variety of matrices and meets the requirements of China, EU MRL and US FDA limits. The Baotai CharmII test uses binding reagents with specific receptor sites. The receptor is added to the sample along with the tracer-labeled antibiotic, and the antibiotic in the sample competes with the tracer-labeled antibiotic for binding sites. The amount of tracer bound to the receptor site is determined and compared to a quality control point. The quality control point is the threshold between negative or positive samples. The greater the amount of tracer measured, the lower the concentration of antibiotic residue in the sample; the smaller the amount of tracer measured, the greater the concentration of antibiotic residue in the sample. Features: The detection limit meets the requirements of MRL in China, EU and FDA in the United States. The detection time is 10-15 minutes and is verified by FDA and "A" pasteurized milk regulations (PMO) in the United States. The same platform to detect a variety of antibiotic residues in different samples is suitable for dairy farms, veterinary stations, laboratories, pastures and control personnel is the national standard and commodity inspection industry standard.

Detection of Baotai Charm Ⅱ Amide Alcohols

The Baotai CharmII Amide Alcohol Test Kit (CAP) can detect chloramphenicol, florfenicol and thiamphenicol in milk. It has been verified by the National Interstate Milk Transportation Conference (NCIMS). In order to detect lower levels of chloramphenicol in milk, the Baotai CharmII Chloramphenicol Test Kit (AIIHM) can be used, with a chloramphenicol detection limit of 0.1ppb. The Pertek CharmII system can also be used to test animal tissue, honey and grain samples. Tissue detection kits can be used to test animal tissues, including beef, pork, and poultry. The grain detection kit can be used to detect grain and feed. The serum detection kit can be used to predict the content of amide alcohol in the tissue of a living animal. Sample pretreatment is simple, milk and urine samples do not need to be extracted, other samples (grain, serum, tissue) need to be extracted simply. Results can be obtained within 10 to 15 minutes. CharmII tests are run on a Bottega CharmII system designed for use by veterinarians, laboratories and regulatory staff. The machine can provide comprehensive analysis of drug residues on a variety of matrices and meets the requirements of China, EU MRL and US FDA limits. The Baotai CharmII test uses binding reagents with specific receptor sites. The receptor is added to the sample along with the tracer-labeled antibiotic, and the antibiotic in the sample competes with the tracer-labeled antibiotic for binding sites. The amount of tracer bound to the receptor site is determined and compared to a quality control point. The quality control point is the threshold between negative or positive samples. The greater the amount of tracer measured, the lower the concentration of antibiotic residue in the sample; the smaller the amount of tracer measured, the greater the concentration of antibiotic residue in the sample. Features: The detection limit of chloramphenicol in milk is 0.1ppb, and that in honey and tissues is 0.3ppb. The detection time is 10-15 minutes. Milk or urine does not need to be extracted from the same platform to detect various antibiotic residues in different samples. It is suitable for dairy farms, veterinary stations, laboratories, pastures and control personnel. Authority: verified by NCIMS in the United States

Detection of Aminoglycosides in Baotai Charm Ⅱ

Po Tai Charm®II aminoglycoside drug detection kit is divided into two kinds, a detection of gentamicin/neomycin drugs, a detection of gentamicin/streptomycin drugs. Sample pretreatment is simple, milk and urine samples do not need to be extracted, other samples (grain, serum, tissue, water) need to be extracted simply. Results can be obtained within 10 to 15 minutes. The Bottega CharmII test runs on a Bottega CharmII system and is designed for use by veterinarians, laboratories and regulatory staff. The machine can provide comprehensive analysis of drug residues on a variety of matrices and meets the requirements of China, EU MRL and US FDA limits. The Baotai CharmII test uses binding reagents with specific receptor sites. The receptor is added to the sample along with the tracer-labeled antibiotic, and the antibiotic in the sample competes with the tracer-labeled antibiotic for binding sites. The amount of tracer bound to the receptor site is determined and compared to a quality control point. The quality control point is the threshold between negative or positive samples. The greater the amount of tracer measured, the lower the concentration of antibiotic residue in the sample; the smaller the amount of tracer measured, the greater the concentration of antibiotic residue in the sample. Features: The detection limit meets the requirements of MRL in China, EU and FDA in the United States. The detection time is 10-15 minutes. Milk or urine does not need to be extracted from the same platform to detect various antibiotic residues in different samples. It is suitable for dairy farms, veterinary stations, laboratories, pastures and control personnel. It is the industry standard for commodity inspection.

Po Tai Charm®SL aflatoxin M1 (qualitative) rapid test strip

Po Tai Charm®SL™Aflatoxin M1 rapid detection strip based on immunochromatography, using ROSA lateral flow technology, qualitative detection of aflatoxin M1 content in milk. The milk sample reacts with the colored microparticles, and after the reaction is complete, the color intensity of the detection zone is read using a ROSA reader with a detection limit of 350ppt. This product can be used in milk stations, farmers, laboratories, fields and prosecutors. Features: The results can be obtained in 3 minutes. The detection limit meets the limit requirements of China and the United States. No sample treatment is required. One-step analysis can be used in conjunction with Baotai CharmEZ antibiotic rapid detection system or ROSA incubator.

Po Tai Charm®MRL Aflatoxin M1 (Quantitative) Rapid Test Strip-Milk

Po Tai Charm®SL™Aflatoxin M1 rapid detection strip based on immunochromatography, using ROSA lateral flow technology, using the EU limit (50ppt) standard, quantitative detection of aflatoxin M1 content in milk. The milk sample reacts with the colored microparticles, and after the reaction is complete, the color intensity of the detection area is read using a ROSA reader and the result is converted to the concentration of aflatoxin M1 in the sample (in ppt). This product can be used in milk stations, farmers, laboratories, fields and prosecutors. Features: The results can be obtained in 15 minutes. The detection limit meets the limit requirements of China and the United States. No sample treatment is required. One-step analysis is certified by the U.S. Environmental Protection Department. It is approved by the Belgian Institute of Agriculture and Fisheries. It can be used in conjunction with the Baotai CharmEZ antibiotic rapid detection system or ROSA incubator.

Po Tai Charm®SL™Aflatoxin M1 (Quantitative) Rapid Test Strip-Milk

Po Tai Charm®SL™Aflatoxin M1 rapid detection strip based on immunochromatography, using ROSA lateral flow technology, using the United States limit (500ppt) standard, quantitative detection of aflatoxin M1 content in milk. The milk sample reacts with the colored microparticles, and after the reaction is complete, the color intensity of the detection area is read using a ROSA reader and the result is converted to the concentration of aflatoxin M1 in the sample (in ppt). This product can be used in milk stations, farmers, laboratories, fields and prosecutors. Features: The results can be obtained in 8 minutes. The detection limit meets the limit requirements of China and the United States. No sample treatment is required. One-step analysis can be used in conjunction with Baotai CharmEZ antibiotic rapid detection system or ROSA incubator.

Baotai Charm ROSA florfenicol and thiamphenicol rapid test strip

Po Tai Charm®Florfenicol and thiamphenicol rapid detection strip, using colloidal gold principle, using ROSA lateral flow technology (rapid one-step detection), rapid detection of florfenicol and thiamphenicol residues in milk. Test results can be interpreted using a ROSA reader or CharmEZ. This product meets the EU or Chinese maximum limit requirements for veterinary drug residues and can be used in milk stations, farmers, laboratories, fields and prosecutors. Features: The results can be obtained in 8 minutes. The detection limit meets the limit requirements of China and the European Union. No sample treatment is required. One-step analysis can be used in conjunction with Baotai CharmEZ antibiotic rapid detection system or ROSA incubator.

Po Tai Charm®Quinolone Rapid Test Strip-Raw and Pasteurized Milk

Po Tai Charm®Quinolone rapid detection strip, using ROSA/lateral flow technology, rapid detection of quinolone residues in milk. Fluoroquinolones are fungicidal drugs used to treat diseases in dairy cows. The detection limit of this product conforms to the European Union MRL standard, and can be used in milk stations, farmers, laboratories, fields and quality control personnel. Features: The results can be obtained in 3 minutes. The detection limit meets the limit requirements of China and the European Union. No sample treatment is required. One-step analysis can be used in conjunction with Baotai CharmEZ antibiotic rapid detection system or ROSA incubator.

Po Tai Charm®Tetracycline Rapid Test Strip

Po Tai Charm®Tetracycline rapid test strip, the use of colloidal gold principle, the use of ROSA®Lateral flow technology (rapid one-step detection) for rapid detection of tetracycline residues in milk. The sensitivity of this product complies with CODEX and EU MRL and Canadian AMRL limit standards, and can be applied to milk stations, farmers, laboratories, fields and prosecutors. Features: The results can be obtained in 8 minutes. The detection limit meets the limit requirements of China and the European Union. No sample treatment is required. One-step analysis can be used in conjunction with Baotai CharmEZ antibiotic rapid detection system or ROSA incubator.

Po Tai Charm®Streptomycin rapid test strip

Po Tai Charm®Streptomycin rapid test strip, the use of colloidal gold principle, the use of ROSA®Lateral flow technology (rapid one-step detection), rapid detection of streptomycin, dihydrostreptomycin residues in milk, the detection limit to meet the United States, the European Union, Canada, Australia, New Zealand, Russia and other countries and Codex Alimentarius requirements. Features: The results can be obtained in 8 minutes. The detection limit meets the limit requirements of China and the European Union. No sample treatment is required. One-step analysis can be used in conjunction with Baotai CharmEZ antibiotic rapid detection system or ROSA incubator.

Po Tai Charm®MRL Sulfonamide Rapid Test Strip

Po Tai Charm®SULF sulfanilamide rapid test strip uses colloidal gold principle and ROSA "lateral flow technology (rapid one-step detection) to rapidly detect sulfanilamide residues in milk, goat milk and sheep milk. The detection limit is 100 μg/kg lower than the EU maximum residue limit. Features: The detection limit can be obtained in 8 minutes to meet the limit requirements of China and EU without sample treatment, one-step analysis can be used with the Baotai CharmEZ Antibiotic Rapid Test System or the ROSA Incubator

Po Tai Charm®Chloramphenicol Rapid Test Strip-Milk

Po Tai Charm®Chloramphenicol rapid detection strip, the use of colloidal gold principle, the use of ROSA®Lateral flow technology (rapid one-step detection) for rapid detection of chloramphenicol residues in milk. This product meets the requirements of the United States and the European Union and can be used in milk stations, farmers, laboratories, fields and prosecutors.

Po Tai Charm®MRL Beta-Lactam/Tetracycline Rapid Test Strip

Po Tai Charm®MRLBLTET rapid test strips, using the principle of immunity, using ROSA-lateral flow technology (rapid one-step detection), rapid detection of β-lactam and tetracycline residues in milk, these include ampicillin, amoxicillin, cefacetonitrile, cephalexin, ceftolonin, cefazolin, cefoperazone, cefquinox, ceftiofur and its metabolites, cefuroxime, cefapirin, cloxacillin, dicloxacillin and penicillin G, as well as chlortetracycline, oxytetracycline and tetracycline from the tetracycline family. The detection limit meets the EU MRL standard and can be applied to milk stations, farmers, laboratories, fields and prosecutors.

Baotai Charm ROSA MRL β-lactam rapid test strip

Po Tai Charm®The detection principle of MRL β-lactam rapid detection strip is immunochromatography, which rapidly detects β-lactam antibiotic residues in milk. The specification is 100 strips per box, the detection time is 8 minutes, and the detection limit meets EU/CODEX limit standard. This kit has passed the official certification of AOAC and FDA, and the certification number Certificate No. 040701.

Baotai Charm ROSA Amide Alcohol Rapid Test Strip

Po Tai Charm®Amide alcohol rapid detection strip, using colloidal gold principle, using ROSA lateral flow technology (rapid one-step detection), rapid detection of amide alcohol residues in milk and muscle. The test results can be interpreted using a ROSA reader or a Baotai Charm EZ. This product meets EU/CODEX or China's maximum limit requirements for veterinary drug residues and can be used in milk stations, farmers, laboratories, fields and prosecutors.

QUAD3 Streptomycin, spectinomycin, kanamycin and neomycin four-in-one test strip

The Baotai Charm QUAD3 test strip uses ROSA rapid one-step immunochromatography technology to quickly detect streptomycin, spectinomycin, kanamycin and neomycin drug residues in milk. The Baotai Charm QUAD3 test strip uses ROSA rapid one-step immunochromatography technology to quickly detect streptomycin, spectinomycin, kanamycin and neomycin drug residues in milk.

A four-in-one test strip for lincomycin, erythromycin, tylosin and gentamicin in the QUAD2-G of Baotai Charm

The Baotai Charm QUAD2-G test strip uses ROSA rapid one-step immunochromatography technology to rapidly detect lincomycin, erythromycin, tylosin and gentamicin drug residues in milk.

Beta-lactams, sulfonamides, quinolones, tetracyclines

The Baotai Charm QUAD1 test strip uses ROSA rapid one-step detection immunochromatography technology to quickly detect β-lactams, tetracyclines, sulfonamides and quinolones residues in milk.

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