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Evergreen Chloramphenicol Rapid Test Kit

Chloramphenicol is a broad-spectrum antibiotic widely used in livestock and poultry breeding, which can kill the main gram-negative bacteria in animals. The European Union prohibits the use of chloramphenicol in food animals. Antibiotic residues in food, such as chloramphenicol, can cause aplastic anemia, which poses a serious threat to public health. Therefore, many countries prohibit the use of chloramphenicol in food animals. Therefore, it is necessary to monitor chloramphenicol in foods such as meat, milk, honey and aquatic products. Evergreen chloramphenicol rapid test kit uses enzyme-linked immunosorbent principle to detect chloramphenicol and chloramphenicol glucuronide in urine and fish and shrimp. Detection principle The kit uses enzyme-linked immunosorbent assay to detect chloramphenicol. The microwells on the ELISA plate provided with the kit were pre-coated with antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. During incubation, both the standard and the sample compete with the enzyme label for the limited binding sites on the coated antibody. Unbound enzyme-labeled conjugate is removed by washing. The substrate reagent is then added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. Kit composition reagent number Chinese name English name specification FB03 standard STANDARD ready-to-use type, 1ml/bottle FB03-008 microplate Microtitreplate12 × 8 wells, coated antibody FB03-009 washing buffer (50 ×)WashBuffer1 × 20ml, if the whole bottle is used, 20ml concentrate 980ml double deionized water, mix well. After dilution, it can be stored at 2-8 ℃ for 30 days. FB03-010 stop solution StopSolution1 mL × 11mL, colorless liquid, transparent bottle, red screw cap, dilute hydrochloric acid solution, ready-to-use FB03-011 substrate solution SubstrateSolution1 mL × 13mL, transparent solution, can be directly used FB03-012 enzyme marker Conjugate1 mL × 11mL, amber bottle, ready-to-use microplate cap PLATECOVER cover microplate instructions IFU

Classification:

Evergreen Veterinary Drug Residues ELISA Kit

Key words:

Biotin

Product number:

BXEFB03A

Specifications:

96 well

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Product Description

EvergreenChloramphenicol Rapid Test KitThe principle of enzyme-linked immunosorbent assay was used to detect chloramphenicol and chloramphenicol glucuronide in urine and fish and shrimp.

Detection principle

The kit used enzyme-linked immunosorbent assay to detect chloramphenicol. The microwells on the ELISA plate provided with the kit were pre-coated with antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. During incubation, both the standard and the sample compete with the enzyme label for the limited binding sites on the coated antibody. Unbound enzyme-labeled conjugate is removed by washing. The substrate reagent is then added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. Use the microplate reader inThe corresponding absorbance values were read at a wavelength of 450 nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated.

Kit composition

Reagent No.	Chinese name	English Name	Specifications
FB03	Standard	STANDARD	ready-to-use, 1ml/bottle
FB03-008	Microplate	Flat Microtitre	12 x 8 wells, coated with antibody
FB03-009	Wash buffer (50x)	Wash Buffer	1 x 20ml, if using the whole bottle, 20ml concentrate 980ml double deionized water, mix well. After dilution, 2-8 ℃ can be stored for 30 days
FB03-010	Stop fluid	Stop Solution	1 × 11mL, colorless liquid, transparent bottle, red screw cap, dilute hydrochloric acid solution, ready-to-use
FB03-011	Substrate solution	Substrate Solution	1 x 13ml, transparent solution, can be used directly
FB03-012	enzyme marker	Conjugate	1 x 11mL, amber bottle, ready-to-use
	Microplate Cover	PLATE COVER	Cover microplate when incubating
	Instructions	IFU