All

All
Product Management
News
Introduction

Home page

Products

Beijing Biotai Co., Ltd. and all staff are dedicate-d to providing customers with the best techni-cal solutions, quality products and after-sales services, and strive to promote the developme-nt of China's food safety industry.

TEL：400-1888-518

Evergreen Streptomycin Assay Kit

The Evergreen streptomycin test kit uses immunological principles to detect streptomycin and dihydrostreptomycin in honey in vitro. Streptomycin is an aminoglycoside antibiotic produced by Streptomyces sp. Streptomycin and dihydrostreptomycin are similar in structure, pharmacokinetics and biological activity. Both drugs are used to treat bacterial infections in cattle, pigs, sheep and poultry. The European Union prohibits the use of streptomycin in beekeeping, while the United States allows its use in the treatment of piglet disease (which requires a four-week holiday). Antibiotics are not allowed to be detected in honey, so it is necessary to monitor streptomycin in honey. Antibiotics in honey can cause allergic reactions and increase drug resistance in humans. Detection principle The kit uses enzyme-linked immunosorbent assay to detect streptomycin. Streptomycin in honey was extracted with dilute wash buffer. The standard or sample extract and the enzyme-labeled conjugate are first added to the microwell, and then the antibody solution is added to start the reaction. During incubation, streptomycin and the enzyme-labeled conjugate in the sample compete for binding to the streptomycin antibody. After incubation, unbound enzyme-labeled conjugate and remaining antibody were removed by washing. The substrate reagent is then added, and the enzyme label reacts with the substrate. After incubation, stop solution was added to stop the reaction and the solution turned yellow. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. Kit composition reagent number Chinese name English name specification FB02 standard STANDARD ready-to-use type, 2ml/bottle FB02-08 microplate Microtitreplate12 × 8 wells, coated antibody binding protein FB02-09 washing buffer (50x) WashBuffer1 × 20ml, if the whole bottle is used, 20ml concentrate 980ml double deionized water, mix well. FB02-10 stop solution StopSolution1 mL × 14mL, colorless liquid, transparent bottle, red screw cap, dilute sulfuric acid solution, ready-to-use FB02-11 substrate solution SubstrateSolution1 mL × 14mL, transparent solution, can directly use FB02-12 enzyme marker Conjugate1 mL × 8mL, ready-to-use FB02-13 antibody solution ANTIBODYSOLUTION1 mL × 8mL, streptomycin antibody (monoclonal), ready-to-use microplate cap PLATECOVER cover microplate instruction IFU

Classification:

Evergreen Veterinary Drug Residues ELISA Kit

Key words:

Biotin

Product number:

BXEFB02A

Specifications:

96 well

Online Inquiry

Product Description

EvergreenStreptomycin test kit uses immunological principles to detect streptomycin and dihydrostreptomycin in honey in vitro. Streptomycin is an aminoglycoside antibiotic produced by Streptomyces sp.Streptomycin and dihydrostreptomycin are similar in structure, pharmacokinetics and biological activity. Both drugs are used to treat bacterial infections in cattle, pigs, sheep and poultry. The European Union prohibits the use of streptomycin in beekeeping, while the United States allows its use in the treatment of piglet disease (which requires a four-week holiday). Antibiotics are not allowed to be detected in honey, so it is necessary to monitor streptomycin in honey. Antibiotics in honey can cause allergic reactions and increase drug resistance in humans.

Detection principle

The kit used enzyme-linked immunosorbent assay to detect streptomycin. Streptomycin in honey was extracted with dilute wash buffer. The standard or sample extract and the enzyme-labeled conjugate are first added to the microwell, and then the antibody solution is added to start the reaction. During incubation, streptomycin and the enzyme-labeled conjugate in the sample compete for binding to the streptomycin antibody. After incubation, unbound enzyme-labeled conjugate and remaining antibody were removed by washing. The substrate reagent is then added, and the enzyme label reacts with the substrate. After incubation, stop solution was added to stop the reaction and the solution turned yellow. Use the microplate reader inThe corresponding absorbance values were read at a wavelength of 450 nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated.

Kit composition

Reagent No.	Chinese name	English Name	Specifications
FB02	Standard	STANDARD	ready-to-use, 2ml/bottle
FB02-08	Microplate	Flat Microtitre	12 x 8 wells, coated with antibody-binding protein
FB02-09	Wash buffer (50x)	Wash Buffer	1 x 20ml, if using the whole bottle, 20ml concentrate 980ml double deionized water, mix well.
FB02-10	Stop fluid	Stop Solution	1 × 14mL, colorless liquid, transparent bottle, red screw cap, dilute sulfuric acid solution, ready-to-use
FB02-11	Substrate solution	Substrate Solution	1 x 14ml, transparent solution, can be used directly
FB02-12	enzyme marker	Conjugate	1 × 8mL, ready-to-use
FB02-13	Antibody solution	ANTIBODY SOLUTION	1 x 8mL, streptomycin antibody (monoclonal), ready-to-use
	Microplate Cover	PLATE COVER	Cover microplate when incubating
	Instructions	IFU