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Zhongjian Baotai

Beijing Biotai Co., Ltd. and all staff are dedicate-d to providing customers with the best techni-cal solutions, quality products and after-sales services, and strive to promote the developme-nt of China's food safety industry.

TEL:400-1888-518

Marine Biotoxins Tests

Neurogenic shellfish toxin (NSP) test

The kit can quantitatively or qualitatively detect the content of short-term naked dinotoxin in water and shellfish samples, and can carry out repeated sample detection on 40 samples, and the detection is fast (time <2 hours), with less sample volume. Product performance detection limit: 0.05ng/mL repeatability: standard coefficient of variation <10%, sample coefficient of variation <15%. Specificity: This kit has no cross-reaction to Saxitoxin, clam toxin, dc-STX, hookweed toxin -1/4, hookweed toxin -2/3, B- 2, B- 1, C- 1/2 and domoic acid. It can cross-react to short naked dinotoxin (PbTx) and its analogs. The cross-reaction rate is as follows: PbTx-3:100 PbTx-2:133 PbTx-5:127% PbTx-2:102% PbTx-9:83% PbTx-6:13% PbTx-1:5% detection principle The kit uses direct competitive enzyme-linked immunosorbent assay to detect the toxin in the sample. The microwells are coated with a short-breve naked dinotoxin antibody, the short-breve naked dinotoxin in the standard/sample competes with the short-breve naked dinotoxin enzyme label for the binding site on the antibody, and the microwells are washed after incubation. Then the substrate reagent is added to react with the enzyme label of the short-term naked dinotoxin, so that the solution turns blue. After the termination solution is added, the solution turns from blue to yellow. The absorbance value is measured at 450nm, and the absorbance value of the unknown sample is compared with the absorbance value of the standard to obtain the concentration of the brevis toxin in the sample. The absorbance value is negatively correlated with the concentration of the brevium toxin in the sample.

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Diarrhetic Shellfish Toxin (DSP) Test

The kit can quantitatively or qualitatively detect okadaic acid in water and shellfish products, and can simultaneously detect 40 samples, with less sample volume and short detection time (less than 2 hours). Product performance detection limit: 0.05ng/mL repeatability: standard coefficient of variation <10%, sample coefficient of variation <15%. Specificity: Diarrheal shellfish toxin detection kit has no cross-reaction to Saxitoxin, Neosaxitoxin, dc-STX, Juncklein -1/4, Juncklein -2/3, B- 2, B- 1, C- 1/2 and domoic acid. It can cross-react to okadaic acid (OA) and DTXs, but the specificity is different, and the cross-reaction rates are as follows. OkadaicAcid(DTX):100% DinophysistoxinsDTX-1:50% DinophysistoxinsDTX-2:50% detection principle The kit uses the direct competitive enzyme-linked immunosorbent assay principle to detect okadaic acid in samples. The micropores were coated with goat anti-rabbit okadaic acid antibody (secondary antibody). Okadaic acid in the standard/sample competes with the okadaic acid enzyme label for the binding site on the okadaic acid antibody, and after being added to the microwell coated with the secondary antibody, the okadaic acid antibody binds to the secondary antibody. The microwells were washed after incubation. Then the substrate reagent is added to react with the okadaic acid enzyme label, so that the solution turns blue. After adding the termination solution, the solution turns from blue to yellow. The concentration of okadaic acid in the sample can be obtained by measuring the absorbance at 450nm and comparing the absorbance of the unknown sample with the absorbance of the standard. The absorbance value is negatively correlated with the concentration of okadaic acid in the sample.

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Detection of Paralytic Shellfish Toxin (PSP)

The kit can quantitatively or qualitatively detect clam toxin in water and shellfish samples, and can carry out duplicate detection on 42 samples, and the detection is fast (time <1 hour) and the sample volume is small. Product performance detection limit: 0.015ng/mL repeatability: standard coefficient of variation <10%, sample coefficient of variation <15%. Specificity: The kit can cross-react with Saxitoxin and analogs, but the specificity is different, and the cross-reaction rate is as follows. Saxitoxin (STX):100% descarbamylated Saxitoxin: 29% GTX2 & 3:23%GTX-5B:23% SulfoGTX1 & 2:2.0%DecarbamoylGTX2 & 3:1.4%Neosaxitoxin clam toxin: 1.3%DecarbamoylNeoSTX:0.6%GTX1 & 4<0.2% detection principle The kit uses direct competitive enzyme-linked immunoassay principle to detect Saxitoxin in samples. The micropores are coated with sheep anti-rabbit stone house toxin antibody (secondary antibody). The Ishifangha toxin in the standard/sample competes with the Ishifangha toxin enzyme label for the binding site on the Ishifangha toxin antibody, and after being added to the micropore coated with the secondary antibody, the Ishifangha toxin antibody binds to the secondary antibody. The microwells were washed after incubation. Then the substrate reagent is added to react with the enzyme label of the stone house toxin, so that the solution turns blue. After the termination solution is added, the solution turns from blue to yellow. The absorbance value is measured at 450nm, and the absorbance value of the unknown sample is compared with the absorbance value of the standard sample to obtain the concentration of Ishifanghaitoxin in the sample. The absorbance value is negatively correlated with the concentration of the toxin in the sample. Kit composition (note: kit stored at 2-8 ℃, do not freeze) Chinese name specification quantity microplate 12, 8 wells/strip 1 plate clam toxin standard 6 bottles of clam toxin enzyme standard 6mL1 bottles of clam toxin antibody solution 6mL, rabbit anti-clam toxin antibody 1 bottle of sample diluent 25mL2 bottles of washing buffer 100mL 1 bottle

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