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Evergreen Beta-Doping Test Kit
Beta-agonists are often used as growth promoters in cattle and swine farming. When animals eat feed, they convert excess energy into fat, but when animals eat beta-stimulants, the excess energy is converted into muscle, not fat. Beta-stimulant is a bronchodilator that opens the airways by relaxing the tense muscles around the airways when asthma or chronic obstructive pulmonary disease attacks. Beta-agonists can cause anxiety, tremors, palpitations or tachycardia, and hypokalemia. The Evergreen beta-stimulant detection kit uses the principle of enzyme-linked immunosorbent assay to detect beta-stimulants in samples. Detection principle The kit uses enzyme-linked immunosorbent assay to detect β-agonists. The microwells on the ELISA plate provided with the kit were pre-coated with antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. During incubation, both the standard and the sample compete with the enzyme label for the limited binding sites on the coated antibody. Unbound enzyme-labeled conjugate is removed by washing. The substrate reagent is then added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. The kit consists of Chinese name English name specification microplate Microtitreplate12 × 8 wells, coated with antibody clenbuterol standard Clenbuterol Standards6 bottle enzyme marker EnzymeConjugate8mL ml/bottle, amber bottle dilution buffer 1 × DiluentionBuffer2 × 25ml, plastic bottle washing solution concentrate (50 ×)WashSolution(50 ×)Concentrate1 × 20ml,50X concentrated substrate solution SubstrateSolution1 × 14mL, amber plastic bottle termination solution StopSolution1 × 8mL
Classification:
Evergreen Veterinary Drug Residues ELISA Kit
Key words:
Product number:
BXEFT05A
Specifications:
96 well
Product Description
Beta-agonists are often used as growth promoters in cattle and swine farming. When animals eat feed, they convert excess energy into fat, but when animals eat beta-stimulants, the excess energy is converted into muscle, not fat. Beta-stimulant is a bronchodilator that opens the airways by relaxing the tense muscles around the airways when asthma or chronic obstructive pulmonary disease attacks. Beta-agonists can cause anxiety, tremors, palpitations or tachycardia, and hypokalemia.
EvergreenThe beta-stimulant detection kit uses the principle of enzyme-linked immunosorbent assay to detect beta-stimulants in samples.
Detection principle
The kit uses enzyme-linked immunosorbent assay to detect β-agonists. The microwells on the ELISA plate provided with the kit were pre-coated with antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. During incubation, both the standard and the sample compete with the enzyme label for the limited binding sites on the coated antibody. Unbound enzyme-labeled conjugate is removed by washing. The substrate reagent is then added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450 nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated.
Kit composition
|
Chinese name |
English Name |
Specifications |
|
Microplate |
Flat Microtitre |
12 x 8 wells, coated with antibody |
|
Clenbuterol Standard |
Clenbuterol Standards |
6 bottles |
|
enzyme marker |
Enzyme Conjugate |
8mL/bottle, amber bottle |
|
Dilution buffer 1x |
Diluention Buffer |
2 x 25ml, plastic bottle |
|
Wash solution concentrate (50 x) |
Wash Solution（50×）Concentrate |
1 x 20ml,50X concentrated |
|
Substrate solution |
Substrate Solution |
1 x 14mL, amber plastic bottle |
|
Stop fluid |
Stop Solution |
1 × 8mL |
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