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Beijing Biotai Co., Ltd. and all staff are dedicate-d to providing customers with the best techni-cal solutions, quality products and after-sales services, and strive to promote the developme-nt of China's food safety industry.

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Evergreen Quinolone Test Kit

Quinolones are indicated for the treatment of community-acquired and nosocomial infections and are bactericidal. It is widely distributed throughout the body with higher tissue permeability than concentrations in plasma, feces, bile, prostate tissue and lung tissue. When the drug is metabolized by the kidneys, the drug penetrates the urine and kidneys. Quinolones have an antibacterial effect on many bacteria, especially gram-negative bacteria. Most side effects are gastrointestinal reactions (nausea, dyspepsia, and vomiting) and central nervous system reactions, such as dizziness, insomnia, and headache. The Biorex Quinolone Assay Kit uses the enzyme-linked immunosorbent principle to detect quinolones (fluoroquinolones) in a sample. Detection principle The kit uses enzyme-linked immunosorbent assay to detect quinolones. The microwells on the ELISA plate provided with the kit were pre-coated with quinolone antibody. A specific enzyme-linked reaction is performed by first adding the standard/sample and the enzyme label to the microwell. During incubation, the quinolones in the standard and sample compete with the enzyme label simultaneously for the limited binding sites on the coated antibody. The microwells are washed and the substrate reagent is added. Enzyme labeling and substrate blue color reaction, after incubation, adding stop solution to stop the reaction, the solution turned yellow. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the quinolone content in the standard/sample, a standard curve is constructed, and the quinolone concentration in the sample is calculated. The kit consists of Chinese name, English name, specification, quinolone microplate Microtitreplate12 × 8 wells, coated with quinolone antibody standard Standards6 × 1.5ml, directly EnzymeConjugate amber bottle with enzyme marker concentrate, 100 μL/bottle high standard SpikingSolution1 × 1mL,100ng/mL washing concentrate WashSolution(50 ×)Concentrate 20mL ,50 × concentrated substrate solution SubstrateSolution amber plastic bottle, 1 × 14mL sample dilution concentrate SampleDiluent10X plastic bottle, blue lid, 2 × 25mL stop liquid StopSolution plastic bottle, red lid, 1 × 9mL microporous lid Platecover

Classification:

Evergreen Veterinary Drug Residues ELISA Kit

Key words:

Biotin

Product number:

BXEFT10A

Specifications:

96 well

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Product Description

EvergreenQuinolone detection kit uses the principle of enzyme-linked immunosorbent assay to detect quinolones (fluoroquinolones) in a sample.

Detection principle

The kit used enzyme-linked immunosorbent assay to detect quinolones. The microwells on the ELISA plate provided with the kit were pre-coated with quinolone antibody. First add the standard to the microwell/Samples and enzyme markers, specific enzyme-linked reaction. During incubation, the quinolones in the standard and sample compete with the enzyme label simultaneously for the limited binding sites on the coated antibody. The microwells are washed and the substrate reagent is added. Enzyme labeling and substrate blue color reaction, after incubation, adding stop solution to stop the reaction, the solution turned yellow. The corresponding absorbance values were read using a microplate reader at a wavelength of 450 nm. The depth of color is inversely proportional to the quinolone content in the standard/sample, a standard curve is constructed, and the quinolone concentration in the sample is calculated.

Kit composition

Chinese name	English Name	Specifications
Quinolone microplate	Flat Microtitre	12 x 8 wells, coated with quinolone antibody
Standard	Standards	6 × 1.5ml, Direct use
Enzyme marker concentrate	Enzyme Conjugate	Amber bottle, 100 μL/bottle
High Standard	Spiking Solution	1 × 1ml, 100ng/ml
washing concentrate	Wash Solution（50×）Concentrate	20mL , 50 x concentrated
Substrate solution	Substrate Solution	Amber plastic bottle, 1 x 14mL
Sample Dilution Concentrate	Sample Diluent 10X	Plastic bottle, blue lid, 2 x 25mL
Stop fluid	Stop Solution	Plastic bottle, red lid, 1 x 9mL
microporous lid	Plate cover