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Evergreen Furaltanone (AMOZ) ELISA Kit
Nitrofuran residues in food pose a serious threat to public health. Nitrofurans are broad-spectrum antibiotics (including nitrofuran, furazolidone, furantoin and furacilin), which have very good antibacterial and pharmacokinetic properties, and have been widely used as growth-promoting additives for poultry, aquaculture and pigs. However, long-term experimental studies have found that nitrofuran drugs and metabolites can cause carcinogenesis and gene mutations in experimental animals. At present, the use of such drugs in animal treatment and feed is prohibited in the United States, Canada and the European Union, and all imported foods containing nitrofurans are prohibited. Therefore, to ensure the health of consumers, antibiotic residues in water sources and food products (such as meat) should be monitored. Detection of nitrofurans is inherently challenging because nitrofurans are rapidly metabolized after ingestion. However, the protein-bound metabolites formed can persist for a long time. AMOZ is the main metabolite of furalitones, which cannot be removed by usual cooking treatments and will be released from tissues under weak acid conditions. Therefore, furalitones metabolites in edible tissues can be monitored and detected. The Biorex Furaltanone (AMOZ) test kit uses the enzyme-linked immunosorbent principle to detect the furaltanone metabolite AMOZ in samples. Detection principle The kit uses enzyme-linked immunosorbent assay to detect furaldone. The microwells on the ELISA plate provided with the kit were pre-coated with AMOZ antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. AMOZ in the standard and sample simultaneously competes with the enzyme label for the limited binding sites on the coated antibody, and the reaction is carried out for 30 minutes. The microwells are washed and the substrate reagent is added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the AMOZ content in the standard/sample, a standard curve is constructed, and the AMOZ concentration in the sample is calculated. The kit consists of Chinese name, English name, specification, AMOZ microplate Microtitreplate12 × 8 wells, coated with AMOZ antibody standard Standards6 × 1.5ml, and directly Furaltadone-HRPConjugateConc with enzyme marker concentrate. 0.2mL enzyme-labeled conjugate diluent ConjugateDiluent12ml, red solution washing solution/sample diluent concentrate WashSolution/SampleDiluent (20x) Concentrate,50ml,20X concentrated AMOZ high-standard AMOZspikingsolution1ml,50ng/ml substrate solution SubstrateSolution14mL stop solution StopSolution8mL
Classification:
Evergreen Veterinary Drug Residues ELISA Kit
Key words:
Product number:
BXEFT03A
Specifications:
96 well
Product Description
Nitrofuran residues in food pose a serious threat to public health. Nitrofurans are broad-spectrum antibiotics (including nitrofuran, furazolidone, furantoin and furacilin), which have very good antibacterial and pharmacokinetic properties, and have been widely used as growth-promoting additives for poultry, aquaculture and pigs. However, long-term experimental studies have found that nitrofuran drugs and metabolites can cause carcinogenesis and gene mutations in experimental animals. At present, the use of such drugs in animal treatment and feed is prohibited in the United States, Canada and the European Union, and all imported foods containing nitrofurans are prohibited. Therefore, to ensure the health of consumers, antibiotic residues in water sources and food products (such as meat) should be monitored.
Detection of nitrofurans is inherently challenging because nitrofurans are rapidly metabolized after ingestion. However, the protein-bound metabolites formed can persist for a long time.AMOZ is the main metabolite of furalitones, which cannot be removed by usual cooking treatments and will be released from tissues under weak acid conditions. Therefore, furalitones metabolites in edible tissues can be monitored and detected.
EvergreenFuralitone (AMOZ) detection kit uses enzyme-linked immunosorbent principle to detect furalitone metabolite AMOZ in samples.
Detection principle
The kit uses enzyme-linked immunosorbent assay to detect furaltanone. The micropores on the enzyme label plate provided by the kit are pre-coated.AMOZ antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. AMOZ in the standard and sample simultaneously competes with the enzyme label for the limited binding sites on the coated antibody, and the reaction is carried out for 30 minutes. The microwells are washed and the substrate reagent is added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450 nm. The depth of color is inversely proportional to the AMOZ content in the standard/sample, a standard curve is constructed, and the AMOZ concentration in the sample is calculated.
Kit composition
|
Chinese name |
English Name |
Specifications |
|
AMOZ microplate |
Flat Microtitre |
12 x 8 wells, coated with AMOZ antibody |
|
Standard |
Standards |
6 × 1.5ml, Direct use |
|
Enzyme marker concentrate |
Furaltadone -HRP Conjugate Conc. |
0.2mL |
|
Dilution of enzyme-labeled conjugate |
Conjugate Diluent |
12ml, red solution |
|
Wash Solution/Sample Diluent Concentrate |
Wash Solution/Sample Diluent（20×）Concentrate， |
50ml,20X concentrated |
|
AMOZ High Standard |
AMOZ spiking solution |
1ml，50ng/ml |
|
Substrate solution |
Substrate Solution |
14mL |
|
Stop fluid |
Stop Solution |
8mL |
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