Products
Beijing Biotai Co., Ltd. and all staff are dedicate-d to providing customers with the best techni-cal solutions, quality products and after-sales services, and strive to promote the developme-nt of China's food safety industry.
TEL：400-1888-518
Evergreen Tetracycline ELISA Kit
Evergreen Tetracycline ELISA Kit is suitable for in vitro quantitative detection of tetracycline in honey and muscle tissue. Tetracycline has antibacterial effect on various gram positive and gram negative bacteria, and it is a kind of broad spectrum antibiotic. Tetracyclines can persist in high concentrations in soil for long periods of time without exhibiting antimicrobial activity. The main effect of tetracyclines on human health is to increase the resistance of human pathogens. In addition, other health effects such as developmental toxicity are also associated with exposure to antibiotics. Detection principle The microwells on the microwell plate provided by the kit are pre-coated with tetracycline antibody. Tetracycline standard/sample and receptor were added to the microplate and incubated. The unbound reagent in the microplate is washed, the enzyme-labeled conjugate is added, and incubated again. The enzyme-labeled conjugate reacts with the receptor bound to the microplate. The microplate was washed again, the substrate reagent was added, and the substrate and the enzyme-labeled conjugate had a blue color reaction. The depth of color is inversely proportional to the amount of tetracycline in the standard/sample. After a period of time, add the stop solution and read the absorbance value using a microplate reader. A standard curve was constructed and the concentration of tetracycline in the sample was calculated. Kit composition 1) microplate: coated tetracycline antibody (vacuum packaged in aluminum foil bag, containing desiccant) 2) tetracycline standard 3) receptor: 3 bottles, freeze-dried powder, 2mL/bottle 4) receptor diluent: 10ml1 bottle 5) concentrated sample dilution buffer (10X):25ml2 bottles, which must be diluted before use 6) concentrated enzyme labeled conjugate: 100 μl,1 bottle 7) enzyme conjugate labeled diluent: 14ml,1 bottle 8) concentrated washing buffer (50X):20ml 9) substrate solution (TMB):14ml 10) stop solution: 6ml 11) microplate cover
Classification:
Evergreen Veterinary Drug Residues ELISA Kit
Key words:
Product number:
BXEFT12A
Specifications:
96 well
Product Description
EvergreenTetracycline ELISA kit is suitable for in vitro quantitative detection of tetracycline in honey and muscle tissue.Tetracycline has antibacterial effect on various gram positive and gram negative bacteria, and it is a kind of broad spectrum antibiotic. Tetracyclines can persist in high concentrations in soil for long periods of time without exhibiting antimicrobial activity. The main effect of tetracyclines on human health is to increase the resistance of human pathogens. In addition, other health effects such as developmental toxicity are also associated with exposure to antibiotics.
Detection principle
The microwells on the microwell plate provided with the kit were pre-coated with tetracycline antibody. Add Tetracycline Standard to Microplate/Samples and receptors, incubation. The unbound reagent in the microplate is washed, the enzyme-labeled conjugate is added, and incubated again. The enzyme-labeled conjugate reacts with the receptor bound to the microplate. The microplate was washed again, the substrate reagent was added, and the substrate and the enzyme-labeled conjugate had a blue color reaction. The depth of color is inversely proportional to the amount of tetracycline in the standard/sample. After a period of time, add the stop solution and read the absorbance value using a microplate reader. A standard curve was constructed and the concentration of tetracycline in the sample was calculated.
Kit composition
1) microplate: coated tetracycline antibody (aluminum foil bag vacuum packaging, containing desiccant)
2) Tetracycline Standard
3) Receptor:3 bottles, freeze-dried powder, 2 mL/bottle after recovery
4) Receptor Diluent:10ml 1 bottle
5) concentrated sample dilution buffer (10X):25ml 2 bottles, must be diluted before use
6) Concentrate enzyme-labeled conjugates:100 μl, 1 bottle
7) Diluent of enzyme-labeled conjugate:14ml, 1 bottle
8) concentrated washing buffer (50X）：20ml
9) Substrate solution (TMB）：14ml
10) Stop fluid:6ml
11) Microplate Cover
Online Inquiry
recommend products
recommend products