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Detection of Paralytic Shellfish Toxin (PSP)
The kit can quantitatively or qualitatively detect clam toxin in water and shellfish samples, and can carry out duplicate detection on 42 samples, and the detection is fast (time <1 hour) and the sample volume is small. Product performance detection limit: 0.015ng/mL repeatability: standard coefficient of variation <10%, sample coefficient of variation <15%. Specificity: The kit can cross-react with Saxitoxin and analogs, but the specificity is different, and the cross-reaction rate is as follows. Saxitoxin (STX):100% descarbamylated Saxitoxin: 29% GTX2 & 3:23%GTX-5B:23% SulfoGTX1 & 2:2.0%DecarbamoylGTX2 & 3:1.4%Neosaxitoxin clam toxin: 1.3%DecarbamoylNeoSTX:0.6%GTX1 & 4<0.2% detection principle The kit uses direct competitive enzyme-linked immunoassay principle to detect Saxitoxin in samples. The micropores are coated with sheep anti-rabbit stone house toxin antibody (secondary antibody). The Ishifangha toxin in the standard/sample competes with the Ishifangha toxin enzyme label for the binding site on the Ishifangha toxin antibody, and after being added to the micropore coated with the secondary antibody, the Ishifangha toxin antibody binds to the secondary antibody. The microwells were washed after incubation. Then the substrate reagent is added to react with the enzyme label of the stone house toxin, so that the solution turns blue. After the termination solution is added, the solution turns from blue to yellow. The absorbance value is measured at 450nm, and the absorbance value of the unknown sample is compared with the absorbance value of the standard sample to obtain the concentration of Ishifanghaitoxin in the sample. The absorbance value is negatively correlated with the concentration of the toxin in the sample. Kit composition (note: kit stored at 2-8 ℃, do not freeze) Chinese name specification quantity microplate 12, 8 wells/strip 1 plate clam toxin standard 6 bottles of clam toxin enzyme standard 6mL1 bottles of clam toxin antibody solution 6mL, rabbit anti-clam toxin antibody 1 bottle of sample diluent 25mL2 bottles of washing buffer 100mL 1 bottle
Classification:
Abraxis shellfish toxin Elisa ELISA Kit
Key words:
Product number:
PN 52255B
Specifications:
96 times
Product Description
The kit can quantitatively or qualitatively detect Saxitoxin in water and shellfish samples.42 samples were tested for duplicate samples, and the test was rapid (time <1Hours), with less sample.
Product Performance
Detection limit:0.015 ng/ml
Repeatability: standard coefficient of variation <10%, sample variation coefficient <15%.
Specificity: The kit can cross-react with Saxitoxin and analogs, but the specificity is different, and the cross-reaction rate is as follows.
Saxitoxin (STX）：100%
Decarbamoyl clam toxin:29%
GTX 2&3:23%
GTX-5B:23%
Sulfo GTX 1 & 2:2.0%
Decarbamoyl GTX 2 & 3:1.4%
NeosaxitoxinClam toxin:1.3%
Decarbamoyl Neo STX:0.6%
GTX 1 & 4 <0.2%
Detection principle
The kit uses the principle of direct competitive enzyme-linked immunosorbent assay to detect Sakotox in samples. The micropores are coated with sheep anti-rabbit stone house toxin antibody (secondary antibody). Standard/The Ishimotoxin in the sample competes with the Ishimotoxin enzyme label for the binding site on the Ishimotoxin antibody, and after being added to the micropore of the coated secondary antibody, the Ishimotoxin antibody binds to the secondary antibody. The microwells were washed after incubation. Then the substrate reagent is added to react with the enzyme label of the stone house toxin, so that the solution turns blue. After the termination solution is added, the solution turns from blue to yellow. In450nmThe concentration of stone house toxin in the sample can be obtained by comparing the absorption value of the unknown sample with the absorption value of the standard. The absorbance value is negatively correlated with the concentration of the toxin in the sample.
Kit composition (Note: Kit2-8℃preserve, do not freeze)
|
Chinese name |
Specifications |
Quantity |
| Microplate | 12Article,8Hole/Article | 1Plate |
| Standard for Saxitoxin | 6Bottle | |
| Saxitoxin enzyme label | 6 mL | 1Bottle |
| Saxitoxin Antibody Solution | 6 mL, Rabbit anti-Sonite toxin antibody | 1Bottle |
| Sample Diluent | 25mL | 2Bottle |
| Wash buffer | 100mL | 1Bottle |
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