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Diarrhetic Shellfish Toxin (DSP) Test
The kit can quantitatively or qualitatively detect okadaic acid in water and shellfish products, and can simultaneously detect 40 samples, with less sample volume and short detection time (less than 2 hours). Product performance detection limit: 0.05ng/mL repeatability: standard coefficient of variation <10%, sample coefficient of variation <15%. Specificity: Diarrheal shellfish toxin detection kit has no cross-reaction to Saxitoxin, Neosaxitoxin, dc-STX, Juncklein -1/4, Juncklein -2/3, B- 2, B- 1, C- 1/2 and domoic acid. It can cross-react to okadaic acid (OA) and DTXs, but the specificity is different, and the cross-reaction rates are as follows. OkadaicAcid(DTX):100% DinophysistoxinsDTX-1:50% DinophysistoxinsDTX-2:50% detection principle The kit uses the direct competitive enzyme-linked immunosorbent assay principle to detect okadaic acid in samples. The micropores were coated with goat anti-rabbit okadaic acid antibody (secondary antibody). Okadaic acid in the standard/sample competes with the okadaic acid enzyme label for the binding site on the okadaic acid antibody, and after being added to the microwell coated with the secondary antibody, the okadaic acid antibody binds to the secondary antibody. The microwells were washed after incubation. Then the substrate reagent is added to react with the okadaic acid enzyme label, so that the solution turns blue. After adding the termination solution, the solution turns from blue to yellow. The concentration of okadaic acid in the sample can be obtained by measuring the absorbance at 450nm and comparing the absorbance of the unknown sample with the absorbance of the standard. The absorbance value is negatively correlated with the concentration of okadaic acid in the sample.
Classification:
Abraxis shellfish toxin Elisa ELISA Kit
Key words:
Product number:
520021
Specifications:
96 times
Product Description
The kit can quantitatively or qualitatively detect okadaic acid in water and shellfish productsand can be detected simultaneously40Samples, less sample volume, short detection time (less2hours).
Product Performance
Detection limit:0.05ng/mL
Repeatability: standard coefficient of variation <10%, sample variation coefficient <15%.
Specificity: Diarrhetic Shellfish Toxin Detection Kit for Saxitoxin, Neosaxitoxin,dc-STX, knee hook algae toxin-1/4Knee hook algae toxin-2/3,B-2,B-1,C- 1/2No cross reaction with domoic acid, okadaic acid (OA）、DTXsCross-reaction can occur, but the specificity is different, the cross-reaction rate is as follows.
Okadaic Acid (DTX) :100%
Dinophysistoxins DTX-1: 50%
Dinophysistoxins DTX-2: 50%
Detection principle
The kit uses the principle of direct competitive enzyme-linked immunosorbent assay to detect okadaic acid in samples. The micropores were coated with goat anti-rabbit okadaic acid antibody (secondary antibody). StandardThe okadaic acid in the sample competes with the okadaic acid enzyme label for the binding site on the okadaic acid antibody, and after being added to the microwell coated with the secondary antibody, the okadaic acid antibody binds to the secondary antibody. The microwells were washed after incubation. Then the substrate reagent is added to react with the okadaic acid enzyme label, so that the solution turns blue. After adding the termination solution, the solution turns from blue to yellow. In450nmThe concentration of okadaic acid in the sample can be obtained by comparing the absorption value of the unknown sample with the absorption value of the standard. The absorbance value is negatively correlated with the concentration of okadaic acid in the sample.
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