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Neurogenic shellfish toxin (NSP) test
The kit can quantitatively or qualitatively detect the content of short-term naked dinotoxin in water and shellfish samples, and can carry out repeated sample detection on 40 samples, and the detection is fast (time <2 hours), with less sample volume. Product performance detection limit: 0.05ng/mL repeatability: standard coefficient of variation <10%, sample coefficient of variation <15%. Specificity: This kit has no cross-reaction to Saxitoxin, clam toxin, dc-STX, hookweed toxin -1/4, hookweed toxin -2/3, B- 2, B- 1, C- 1/2 and domoic acid. It can cross-react to short naked dinotoxin (PbTx) and its analogs. The cross-reaction rate is as follows: PbTx-3:100 PbTx-2:133 PbTx-5:127% PbTx-2:102% PbTx-9:83% PbTx-6:13% PbTx-1:5% detection principle The kit uses direct competitive enzyme-linked immunosorbent assay to detect the toxin in the sample. The microwells are coated with a short-breve naked dinotoxin antibody, the short-breve naked dinotoxin in the standard/sample competes with the short-breve naked dinotoxin enzyme label for the binding site on the antibody, and the microwells are washed after incubation. Then the substrate reagent is added to react with the enzyme label of the short-term naked dinotoxin, so that the solution turns blue. After the termination solution is added, the solution turns from blue to yellow. The absorbance value is measured at 450nm, and the absorbance value of the unknown sample is compared with the absorbance value of the standard to obtain the concentration of the brevis toxin in the sample. The absorbance value is negatively correlated with the concentration of the brevium toxin in the sample.
Classification:
Abraxis shellfish toxin Elisa ELISA Kit
Key words:
Product number:
520026
Specifications:
96 times
Product Description
The kit can quantitatively or qualitatively detect the content of short naked dinotoxin in water and shellfish samples.40 samples were tested for duplicate samples, and the test was rapid (time <2Hours), with less sample.
Product Performance
Detection limit:0.05 ng/ml
Repeatability: standard coefficient of variation <10%, sample variation coefficient <15%.
Specificity: This kit is sensitive to Saxitoxin, clam toxin,dc-STX, knee hook algae toxin-1/4Knee hook algae toxin-2/3,B-2,B-1,C- 1/2No cross-reactivity with domoic acid
To the toxin of the brevium (PbTx) and its analogs can cross-react, and the cross-reaction rate is as follows:
PbTx-3:100%
deoxidationPbTx-2 ：133%
PbTx-5:127%
PbTx-2:102%
PbTx-9:83%
PbTx-6:13%
PbTx-1:5%
Detection principle
The kit uses the principle of direct competitive enzyme-linked immunosorbent assay to detect the breve dinotoxin in the sample. Microwell coated with nudedinotoxin antibody, standard/The breviotoxin in the sample competes with the breviotoxin enzyme label for the binding site on the antibody, and the microwells are washed after incubation. Then the substrate reagent is added to react with the enzyme label of the short-term naked dinotoxin, so that the solution turns blue. After the termination solution is added, the solution turns from blue to yellow. In450nmThe concentration of short naked algae toxin in the sample can be obtained by measuring the absorbance value of the unknown sample and comparing the absorbance value of the standard. The absorbance value is negatively correlated with the concentration of the brevium toxin in the sample.
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