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Evergreen Ractopamine Test Kit
Ractopamine is a synthetic beta adrenoceptor agonist. When animals eat ractopamine, it increases animal growth rate and lean meat percentage. Beta agonists, such as ractopamine, cause an increase in the animal's heart rate. The Evergreen ractopamine detection kit uses the enzyme-linked immunosorbent principle to detect ractopamine in urine, tissue, feed and milk. Detection principle The microwells on the enzyme plate provided by the kit are pre-coated with antibodies. A standard/sample solution, an enzyme label are added to the microwells, and during incubation, ractopamine in the standard/sample simultaneously competes with the enzyme label for the limited binding sites on the coated antibody. Unbound enzyme-labeled conjugate is removed by washing. The substrate reagent is then added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. Kit composition Chinese name English name specification premixed microplate PremixingMicrotiter12 × 8 wells, uncoated microplate Microtitreplate12 × 8 wells, coated ractopamine antibody ractopamine standard Ractopamine Standards7 bottle enzyme marker Conjugate1 × 0.2mL/bottle, plastic bottle enzyme marker diluent Conjugatediluent1 × 30mL dilution buffer (10 ×)DilutionBuffer 10 × 2 × 25mL, plastic bottle substrate concentrate Chromogen1 × 3mL substrate buffer Chromogenbuffer1 × 24mL, glass bottle washing solution concentrate (50 ×)WashSolution(50 ×)Concentrate1 × 20ml,50X concentrated stop solution StopSolution1 × 8mL
Classification:
Evergreen Veterinary Drug Residues ELISA Kit
Key words:
Product number:
BXEFT11A
Specifications:
96 well
Product Description
Ractopamine is a syntheticBeta adrenoceptor agonists. When animals eat ractopamine, it increases animal growth rate and lean meat percentage. Beta agonists, such as ractopamine, cause an increase in the animal's heart rate.
EvergreenRactopamine Test KitEnzyme-linked immunosorbent assay was used to detect ractopamine in urine, tissue, feed and milk.
Detection principle
The microwells on the ELISA plate provided with the kit were pre-coated with antibody. Add standard to microwells/sample solution, enzyme label, during incubation, ractopamine in the standard/sample simultaneously competes with the enzyme label for the limited binding sites on the coated antibody. Unbound enzyme-labeled conjugate is removed by washing. The substrate reagent is then added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450 nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated.
Kit composition
|
Chinese name |
English Name |
Specifications |
|
Premixed Microplate |
Premixing Microtiter |
12 x 8 wells, uncoated |
|
Microplate |
Flat Microtitre |
12 x 8 wells, coated with ractopamine antibody |
|
Ractopamine Standard |
Ractopamine Standards |
7 bottles |
|
enzyme marker |
Conjugate |
1 x 0.2mL/bottle, plastic bottle |
|
enzyme marker dilution |
Conjugate diluent |
1 × 30mL |
|
Dilution buffer (10x) |
Dilution Buffer 10 x |
2 x 25mL, plastic bottle |
|
Substrate concentrate |
Chromogen |
1 × 3mL |
|
Substrate buffer |
Chromogen buffer |
1 x 24mL, glass bottle |
|
Wash solution concentrate (50 x) |
Wash Solution(50×)Concentrate |
1 x 20ml,50X concentrated |
|
Stop fluid |
Stop Solution |
1 × 8mL |
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