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Zhongjian Baotai Biotin Test Kit
The biotin detection kit uses a microbial method to quantitatively detect the total amount of biotin (added biotin and native biotin) in food, feed and medicine. The kit contains all the reagents and standards required for the experiment, and the kit can perform 96 assays. Biotin detection kit is a microbial method for quantitative detection of the total amount of biotin in food, feed and medicine. The microbiological detection method follows AOAC international norms and conforms to national standards. The culture medium and the diluted sample extract are added to the microwells coated with Lactobacillus plantarum (Lactobacillusplantarum), and the Lactobacillus plantarum will grow until the biotin in the culture liquid is completely consumed. 37 C, dark, incubated for 44-48 hours. The growth status (turbidity) of Lactobacillus plantarum was positively correlated with the biotin content, and the sample turbidity was read at 610-630nm or 540-550nm wavelength by using a microplate reader, and the biotin content was obtained by comparing the turbidity of the sample with the standard curve. Product features: trust: based on a variety of official reference methods (AOAC,EU & LFGB,GB) research and development, high accuracy (CV<10%). Simple: kit microwell plate package bacteria, no need to purchase. Samples can be extracted in a hot water bath. Fast: The incubation time is 24 to 44 hours, which is 3-5 days less than traditional methods. Product performance: recovery rate: 90-105% repeatability: intra-batch difference <10%; Inter-batch difference <10%
Classification:
Zhongjian Baotai Vitamin Test Kit
Key words:
Product number:
HC10310
Specifications:
96 well
Product Description
The biotin detection kit uses a microbial method to quantitatively detect the total amount of biotin (added biotin and native biotin) in food, feed and medicine. The kit contains all the reagents and standards required for the experiment, and the kit can perform 96 assays.
Biotin detection kit is a microbial method for quantitative detection of the total amount of biotin in food, feed and medicine. The microbial detection method followsAOAC international norms, in line with national standards. Add the culture medium and diluted sample extract to the lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus plantarum will grow until the biotin in the culture solution is completely consumed. 37 ℃, dark, incubated44-48 hours. The growth status (turbidity) of Lactobacillus plantarum was positively correlated with the biotin content, and the sample turbidity was read at 610-630nm or 540-550nm wavelength by using a microplate reader, and the biotin content was obtained by comparing the turbidity of the sample with the standard curve.
Product features:
Trust: developed according to various official reference methods (AOAC, EU & LFGB,GB) with high accuracy (CV<10%).
Simple: kit microwell plate package bacteria, no need to purchase. Samples can be extracted in a hot water bath.
Fast: The incubation time is 24 to 44 hours, which is 3-5 days less than traditional methods.
Product performance:
Recovery:90-105%
repeatability: intra-batch variance<10%; inter-lot variance<10%
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