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Evergreen Fumisin Assay Kit
Fumonisin (B1, B2, B3) is a group of mycotoxins produced by Fusarium moniliforme. It widely exists in nature, mainly contaminates corn, and can cause liver cancer in rats, pulmonary edema in pigs and leukomalacia in horses. In South Africa and China, areas with high levels of fumonisins in maize are areas of high incidence of esophageal cancer. Evergreen fumatoxin detection kit can quantitatively detect the content of fumatoxin in grain and feed. Detection principle Evergreen fumosin detection kit uses solid-phase direct competitive enzyme-linked immunosorbent principle to detect fumosin in samples. Microwells are coated with a fumonisin-specific monoclonal antibody that cross-reacts with three subtypes of fumonisin. The fumonisin in the standard/sample competes with the fumonisin enzyme label for binding to the binding site on the antibody. After completion of the reaction, the plate was washed to remove non-specific reactants. Substrate reagent (TMB) is added to the microwell, and the solution turns blue, with color intensity proportional to the concentration of the fumarotoxin enzyme label and inversely proportional to the concentration of the fumarotoxin in the sample or standard. The stop solution was then added and the color changed from blue to yellow. Use a microplate reader to detect the OD value of the solution in the micropore at 450nm, and compare the OD value of the sample with the OD value of the standard, and the result can be obtained. The kit consists of Chinese name, English name, specification and quantity of micropores. The Antibodycoatedmicrowells are placed in a microplate, with a total of 96 micropores (12, 8 wells/strip). One bag of dilution wells(green) coated with mouse anti-fumonisin monoclonal antibody is Dilutionwells(green) and placed in a microplate, with a total of 96 micropores, uncoated antibody (12 bars, 8 holes/bar) 1 plate of fumaritoxin standard FumonisinStandards fumaritoxin aqueous solution, 1.5mL6 bottles of fumaritoxin enzyme standard per bottle FumonisinHRPconjugate the conjugate of fumaritoxin and peroxidase, fumaritoxin enzyme standard dissolved in preservative-containing buffer solution, 12mL2 bottles of substrate reagent SubstrateReagent15mL per bottle, 1 bottle of stable tetramethylbenzidine (TMB) termination liquid StopSolution15mL, acid solution 1 bottle of washing buffer Washingbuffer PBS buffer containing 0.05% Tween 20, diluted to 1L with distilled water before use, refrigerated 1 bag
Classification:
Evergreen Mycotoxin ELISA Kit
Key words:
Product number:
EB10610
Specifications:
96 well
Product Description
Fumotoxin (B1, B2, B3) is a group of mycotoxins produced by Fusarium moniliforme. It widely exists in nature and mainly pollutes corn, which can cause liver cancer in mice, pulmonary edema in pigs and leukomalacia in horses. In South Africa and China, areas with high levels of fumonisins in maize are areas of high incidence of esophageal cancer.
Evergreen fumatoxin detection kit can quantitatively detect the content of fumatoxin in grain and feed.
Detection principle
The Evergreen fumarotoxin detection kit uses the solid-phase direct competitive enzyme-linked immunosorbent principle to detect fumarotoxin in the sample. Microwells are coated with a fumonisin-specific monoclonal antibody that cross-reacts with three subtypes of fumonisin. The fumonisin in the standard/sample competes with the fumonisin enzyme label for binding to the binding site on the antibody. After completion of the reaction, the plate was washed to remove non-specific reactants. Substrate reagent (TMB) is added to the microwell, and the solution turns blue, with color intensity proportional to the concentration of the fumarotoxin enzyme label and inversely proportional to the concentration of the fumarotoxin in the sample or standard. The stop solution was then added and the color changed from blue to yellow. Use a microplate reader to detect the OD value of the solution in the micropore at 450nm, and compare the OD value of the sample with the OD value of the standard, and the result can be obtained.
Kit composition
|
Chinese name |
English Name |
Specifications |
Quantity |
|
Micropores |
Antibody coated microwells |
Placed in a microplate, a total of 96 microwells (12, 8 wells/strip), coated with mouse anti-fumonisin monoclonal antibody |
1 bag |
|
Dilution hole (green) |
Dilution wells (green) |
Placed in a microplate, a total of 96 microwells, uncoated with antibody (12, 8 wells/strip) |
1 Plate |
|
Fumotoxin Standard |
Fumonisin Standards |
Fumotoxin aqueous solution, 1.5mL per bottle |
6 bottles |
|
Fumisin enzyme marker |
Fumonis HRP conjugate. |
Conjugate of fumisin and peroxidase, fumisin enzyme label in buffer containing preservative, 12mL per bottle |
2 bottles |
|
substrate reagent |
Substrate Reagent |
15mL, stabilized tetramethylbenzidine (TMB) |
1 bottle |
|
Stop fluid |
Stop Solution |
15mL, acidic solution |
1 bottle |
|
Wash buffer |
Washing buffer |
PBS buffer containing 0.05% Tween 20, diluted to 1L with distilled water before use, refrigerated |
1 bag |
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