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Evergreen Zearalenone Rapid Test Kit
Zearalenone (ZEA) is a metabolite of Fusarium and Gibberella spp. Zearalenone contamination is very common, like corn, wheat, oats, barley and sorghum are very easy to contaminate zearalenone. Ingestion of zearalenone by animals, particularly pigs, can lead to infertility, miscarriage or other reproductive problems. TREATM zearalenone detection kit can quantitatively determine zearalenone in corn and wheat samples, which is rapid, sensitive and reliable. The kit contains all the reagents required for the enzyme-linked immunoassay, and the kit can perform 96 tests (including standards). Detection principle EvergreenTREATM zearalenone detection kit is a competitive enzyme-linked immunoassay (EISA). Zearalenone standard/sample and zearalenone antibody are added to the microwells coated with zearalenone antigen, free zearalenone and zearalenone antigen coated in the microwells compete for binding sites on the zearalenone antibody, and unbound zearalenone and zearalenone antibodies are removed during the first wash. Horseradish peroxidase-labeled secondary antibody (HRP) is then added and unbound enzyme-labeled conjugate is removed in a second washing step. Substrate reagent is then added to each well and the bound enzyme conjugate catalyzes the substrate solution to turn blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of zearalenone in the sample or standard. The kit consists of Chinese name, English name, specification, quantity, microplate Microtiterplate, a total of 96 micropores (12, 8 wells/strip), coated with ZEA-OVA1 bags of standard solution Standardssolutions0ppb,2ml2ppb,5ppb,10ppb,25ppb,100ppb, 1mL per bottle, ready-to-use. 6 bottles of sample diluent SampleDiluent15ml, white cap, ready-to-use type 1 bottle of antibody solution Anti-ZEA antibody6ml, white cap, ready-to-use type. 1 bottle of substrate TMBSubstrate12mL, brown cap, stable tetramethylbenzidine (TMB), ready-to-use 1 bottle of enzyme-labeled conjugate HRPConjugate6ml, green cap, ready-to-use 1 bottle of stop solution StopSolution6mL, red cap, containing 0.18M sulfuric acid, ready-to-use 1 bottle of washing solution WashSolution60ml,20 times concentrated 1 bottle
Classification:
Evergreen Mycotoxin ELISA Kit
Key words:
Product number:
EB10412R
Specifications:
96 well
Product Description
Zearalenone (ZEA）It is a metabolite of Fusarium and Gibberella. Zearalenone contamination is very common, like corn, wheat, oats, barley and sorghum are very easy to contaminate zearalenone. Ingestion of zearalenone by animals, particularly pigs, can lead to infertility, miscarriage or other reproductive problems.
TREATMZearalenone detection kit can be used for quantitative determination of zearalenone in corn and wheat samples, which is rapid, sensitive and reliable. The kit contains all the reagents required for the enzyme-linked immunoassay, and the kit can perform 96 tests (including standards).
Detection principle
Evergreen TREATMZearalenone detection kit is a competitive enzyme-linked immunoassay (EISA). Zearalenone standard/sample and zearalenone antibody are added to the microwells coated with zearalenone antigen, free zearalenone and zearalenone antigen coated in the microwells compete for binding sites on the zearalenone antibody, and unbound zearalenone and zearalenone antibodies are removed during the first wash. Horseradish peroxidase-labeled secondary antibody (HRP) is then added and unbound enzyme-labeled conjugate is removed in a second washing step. Substrate reagent is then added to each well and the bound enzyme conjugate catalyzes the substrate solution to turn blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of zearalenone in the sample or standard.
Kit composition
|
Chinese name |
English Name |
Specifications |
Quantity |
|
Microplate |
Microtiter plate |
A total of 96 micropores (12, 8 wells/strip), coated with ZEA-OVA |
1 bag |
|
Standard solution |
Standards solutions |
0ppb，2ml 2ppb,5ppb,10ppb,25ppb,100ppb, 1mL per bottle, ready to use. |
6 bottles |
|
Sample Diluent |
Sample Diluent |
15ml, white cover, ready-to-use type |
1 bottle |
|
Antibody solution |
Anti-ZEA antibody |
6ml, white cover, ready to use. |
1 bottle |
|
Substrate |
TMB Substrate |
12 mL, brown cap, stabilized tetramethylbenzidine (TMB), ready-to-use |
1 bottle |
|
Enzyme-labeled conjugates |
HRP Conjugate |
6ml, green cover, ready-to-use |
1 bottle |
|
Stop fluid |
Stop Solution |
6 mL, red cover, containing 0.18M sulfuric acid, ready-to-use |
1 bottle |
|
Wash solution |
Wash Solution |
60ml ,20 times concentrated |
1 bottle |
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