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Evergreen Aflatoxin B1 Highly Sensitive Kit-Milk & Dairy Products
Aflatoxins are toxic metabolites produced by a variety of molds (such as Aspergillus flavus and Aspergillus parasiticus) and are highly carcinogenic. Aflatoxin B1 is usually present in cereals, cottonseeds and nuts at the same time as aflatoxins B2, G1, G2. EvergreenTREATM Aflatoxin B1 high sensitive detection kit was used to quantitatively detect aflatoxin B1 in milk and milk by competitive enzyme-linked immunosorbent assay. The kit contains all reagents required for the reaction-including standards, enough for 96 assays (including standards). Quantitative detection requires a microplate reader. Detection principle Aflatoxin B1 test kit is based on antigen-antibody reaction. The microwells are coated with an antigen that can bind to the aflatoxin B1 antibody. Standard or sample and enzyme labeled antibody are added to the microwells. Free aflatoxin and antigen coated in the microwell compete for binding to the binding site of the enzyme-labeled antibody. Unbound enzyme-labeled antibody is removed in a washing step. Substrate reagent was then added and the solution turned blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of aflatoxin B1 in the sample or standard. The kit consists of Chinese name, English name, specification, quantity, microplate MicroteterPlate, 96 micropores (12, 8 wells/strip), 1 bag of standard solution Standards solutions coated with antigen, 1mL per bottle, free aflatoxin B1, ready-to-use 6 bottles of sample diluent SampleDiluent15ml, white cap, ready-to-use 1 bottle of substrate TMBSubstrate12 mL, brown cap, stable tetramethylbenzidine (TMB), the ready-to-use 1 bottle of enzyme-labeled conjugate HRPConjugate6ml, green cap, ready-to-use 1 bottle of stop solution StopSolution6 mL, red cap, containing 0.18M sulfuric acid, ready-to-use 1 bottle of washing solution WashSolution60ml,20 times concentrated 1 bottle
Classification:
Evergreen Mycotoxin ELISA Kit
Key words:
Product number:
EB10211H
Specifications:
96 well
Product Description
Aflatoxins are toxic metabolites produced by a variety of molds (such as Aspergillus flavus and Aspergillus parasiticus) and are highly carcinogenic. aflatoxinB1 is usually present in cereals, cottonseeds and nuts at the same time as aflatoxins B2, G1, G2.
Evergreen TREATMaflatoxinThe B1 highly sensitive detection kit uses the principle of competitive enzyme-linked immunosorbent assay to quantitatively detect aflatoxin B1 in milk and milk.The kit contains all the reagents required for the reaction-Standards included, enough for 96 tests (including standards). Quantitative detection requires a microplate reader.
Detection principle
aflatoxinThe B1 test kit is based on an antigen-antibody reaction. The microwells are coated with an antigen that can bind to the aflatoxin B1 antibody. Standard or sample and enzyme labeled antibody are added to the microwells. Free aflatoxin and antigen coated in the microwell compete for binding to the binding site of the enzyme-labeled antibody. Unbound enzyme-labeled antibody is removed in a washing step. Substrate reagent was then added and the solution turned blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of aflatoxin B1 in the sample or standard.
Kit composition
|
Chinese name |
English Name |
Specifications |
Quantity |
|
Microplate |
Microteter Plate |
A total of 96 microwells (12, 8 wells/strip), coated with antigen |
1 bag |
|
Standard solution |
Standards solutions |
1mL per bottle, free aflatoxin B1, ready-to-use |
6 bottles |
|
Sample Diluent |
Sample Diluent |
15ml, white cover, ready-to-use type |
1 bottle |
|
Substrate |
TMB Substrate |
12 mL, brown cap, stabilized tetramethylbenzidine (TMB), ready-to-use |
1 bottle |
|
Enzyme-labeled conjugates |
HRP Conjugate |
6ml, green cover, ready-to-use |
1 bottle |
|
Stop fluid |
Stop Solution |
6 mL, red cover, containing 0.18M sulfuric acid, ready-to-use |
1 bottle |
|
Wash solution |
Wash Solution |
60ml,20 times concentrated |
1 bottle |
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