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Evergreen Aflatoxin M1 Rapid Test Kit
Aflatoxins are toxic secondary metabolites produced by a variety of molds (such as Aspergillus flavus and Aspergillus parasiticus) and are highly carcinogenic. Aflatoxins are commonly found in grains, nuts, and other crop products. Aflatoxins are potential carcinogens and immunosuppressants. Aflatoxins have different subtypes, with B1 being the most common and the most toxic. When cows ingest contaminated feed, aflatoxin B1 occurs hydroxylation reaction to produce aflatoxin M1, aflatoxin M1 can enter the milk with lactation. Aflatoxin M1 is stable throughout the bus disinfection process and can enter the final product for human consumption. Most countries around the world have established limits for aflatoxin M1 in milk and dairy products. The US and EU set a limit of 50ppt for aflatoxin M1 in milk and reconstituted milk powder. The TREA Aflatoxin M1 Rapid Test Kit can detect aflatoxin M1 in milk and dairy products qualitatively or quantitatively. The kit contains all reagents required for ELISA experiments, including standards. The kit can perform 96 tests. Scope of application Evergreen aflatoxin M1 rapid test kit is suitable for qualitative or quantitative detection of aflatoxin M1 in milk or milk products. 2. Detection principle TREA aflatoxin M1 detection kit is based on antigen-antibody reaction. The microwells were coated with aflatoxin M1 antibody. The aflatoxin standard or sample and the enzyme label conjugate are added to the microwells. Free aflatoxin M1 and the enzyme-labeled conjugate compete for binding to the antibody in the microwell. Unbound enzyme-labeled conjugate is removed in a washing step. Substrate reagent was then added and the solution turned blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of aflatoxin M1 in the sample or standard. 3. Kit Composition Enough reagents for 96 reactions were included in the kit. Chinese name English name specification quantity microplate AflatoxinM1plate a total of 96 microholes (12, 8 wells/strip), coated with aflatoxin M1 antibody 1 bag of standard solution Standards solutions 1mL per bottle, ready-to-use 6 bottles of substrate TMBSubstrate12mL, brown cap, stable tetramethylbenzidine (TMB), ready-to-use 1 bottle of enzyme-labeled HRPConjugate6mL, green cap, horseradish peroxidase labeled aflatoxin, one bottle of ready-to-use stop solution StopSolution6 mL, red cap, containing 0.18M sulfuric acid, one bottle of ready-to-use washing solution WashSolution60ml20-fold concentrated, one bottle of sample diluent SampleDiluent50mL, one bottle of ready-to-use
Classification:
Evergreen Mycotoxin ELISA Kit
Key words:
Product number:
EB10311H
Specifications:
96 well
Product Description
Aflatoxins are toxic secondary metabolites produced by a variety of molds (such as Aspergillus flavus and Aspergillus parasiticus) and are highly carcinogenic. Aflatoxins are commonly found in grains, nuts, and other crop products. Aflatoxins are potential carcinogens and immunosuppressants. Aflatoxins have different subtypes, whichB1 is the most common and most toxic. When cows ingest contaminated feed, aflatoxin B1 occurs hydroxylation reaction to produce aflatoxin M1, aflatoxin M1 can enter the milk with lactation. Aflatoxin M1 is stable throughout the bus disinfection process and can enter the final product for human consumption. Most countries around the world have established limits for aflatoxin M1 in milk and dairy products. The US and EU set a limit of 50ppt for aflatoxin M1 in milk and reconstituted milk powder.
The TREA Aflatoxin M1 Rapid Test Kit can detect aflatoxin M1 in milk and dairy products qualitatively or quantitatively. The kit contains all reagents required for ELISA experiments, including standards. The kit can perform 96 tests.
Scope of application
Evergreen Aflatoxin M1 Rapid Test Kit is suitable for qualitative or quantitative detection of aflatoxin M1 in milk or milk products.
2. Detection principle
The TREA Aflatoxin M1 test kit is based on antigen-antibody reactions. The microwells were coated with aflatoxin M1 antibody. The aflatoxin standard or sample and the enzyme label conjugate are added to the microwells. Free aflatoxin M1 and the enzyme-labeled conjugate compete for binding to the antibody in the microwell. Unbound enzyme-labeled conjugate is removed in a washing step. Substrate reagent was then added and the solution turned blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of aflatoxin M1 in the sample or standard.
3. Kit composition
The kit containsEnough reagents for 96 reactions.
|
Chinese name |
English Name |
Specifications |
Quantity |
|
Microplate |
Aflatoxin M1 plate |
A total of 96 microwells (12, 8 wells/strip) coated with aflatoxin M1 antibody |
1 bag |
|
Standard solution |
Standards solutions |
1mL per bottle, ready to use |
6 bottles |
|
Substrate |
TMB Substrate |
12mL, brown cap, stabilized tetramethylbenzidine (TMB), ready-to-use |
1 bottle |
|
Enzyme-labeled conjugates |
HRP Conjugate |
6mL, green cap, horseradish peroxidase labeled aflatoxin, ready-to-use type |
1 bottle |
|
Stop fluid |
Stop Solution |
6 mL, red cover, containing 0.18M sulfuric acid, ready-to-use |
1 bottle |
|
Wash solution |
Wash Solution |
60ml 20 times concentrated |
1 bottle |
|
Sample Diluent |
Sample Diluent |
50 mL, ready-to-use |
1 bottle |
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