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ELISA Kit for Evergreen Furazolidone (AOZ)

Nitrofuran residues in food pose a serious threat to public health. Nitrofurans are broad-spectrum antibiotics (including nitrofuran, furazolidone, furantoin and furacilin), which have very good antibacterial and pharmacokinetic properties, and have been widely used as growth-promoting additives for poultry, aquaculture and pigs. However, long-term experimental studies have found that nitrofuran drugs and metabolites can cause carcinogenesis and gene mutations in experimental animals. At present, the use of such drugs in animal treatment and feed is prohibited in the United States, Canada and the European Union, and all imported foods containing nitrofurans are prohibited. Therefore, to ensure the health of consumers, antibiotic residues in water sources and food products (such as meat) should be monitored. Detection of nitrofurans is inherently challenging because nitrofurans are rapidly metabolized after ingestion. However, the protein-bound metabolites formed can persist for a long time. AOZ is the main metabolite of furazolidone, which cannot be removed by usual cooking treatments and will be released from tissues under weak acid conditions, so furazolidone metabolites in edible tissues can be monitored and detected. The Biorex Furazolidone (AOZ) test kit uses the principle of enzyme-linked immunosorbent assay to detect the furazolidone metabolite AOZ in a sample. Detection principle The kit uses enzyme-linked immunosorbent assay to detect furazolidone. The micropores on the ELISA plate provided with the kit were pre-coated with AOZ antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. The AOZ in the standard and sample simultaneously competes with the enzyme label for the limited binding sites on the coated antibody, and the reaction is carried out for 30 minutes. The microwells are washed and the substrate reagent is added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the AOZ content in the standard/sample, a standard curve is constructed, and the AOZ concentration in the sample is calculated. The kit consists of Chinese name, English name, specification, AOZ microplate Microtitreplate12 × 8 wells, coated with AOZ antibody standard Standards6 × 1.5ml, and directly Furazolidone-HRPConjugateConc with enzyme marker concentrate. 0.2mL enzyme-labeled conjugate diluent ConjugateDiluent12ml, red solution washing solution/sample diluent concentrate WashSolution/SampleDiluent (20x) Concentrate,50ml,20X concentrated AOZ high-standard AOZspikingsolution1ml,50ng/ml substrate solution SubstrateSolution14mL stop solution StopSolution8mL microplate lid PlateCover

Classification:

Evergreen Veterinary Drug Residues ELISA Kit

Key words:

Biotin

Product number:

BXEFT04A

Specifications:

96 well

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Product Description

Detection of nitrofurans is inherently challenging because nitrofurans are rapidly metabolized after ingestion. However, the protein-bound metabolites formed can persist for a long time.AOZ is the main metabolite of furazolidone, which cannot be removed by usual cooking treatments and will be released from tissues under weak acid conditions, so furazolidone metabolites in edible tissues can be monitored and detected.

EvergreenFurazolidone (AOZ) detection kit uses the principle of enzyme-linked immunosorbent assay to detect the furazolidone metabolite AOZ in samples.

Detection principle

The kit used enzyme-linked immunosorbent assay to detect furazolidone. The micropores on the enzyme label plate provided by the kit are pre-coated.AOZ antibody. The standard and derivatized samples are added to the microwell first, and then the enzyme label is added to perform a specific enzyme-linked reaction. The AOZ in the standard and sample simultaneously competes with the enzyme label for the limited binding sites on the coated antibody, and the reaction is carried out for 30 minutes. The microwells are washed and the substrate reagent is added. The enzyme label reacts with the substrate, and the reaction is terminated by adding a stop solution after incubation. The corresponding absorbance values were read using a microplate reader at a wavelength of 450 nm. The depth of color is inversely proportional to the AOZ content in the standard/sample, a standard curve is constructed, and the AOZ concentration in the sample is calculated.

Kit composition

Chinese name	English Name	Specifications
AOZ microplate	Flat Microtitre	12 x 8 wells, coated with AOZ antibody
Standard	Standards	6 × 1.5ml, Direct use
Enzyme marker concentrate	Furazolidone-HRP Conjugate Conc.	0.2mL
Dilution of enzyme-labeled conjugate	Conjugate Diluent	12ml, red solution
Wash Solution/Sample Diluent Concentrate	Wash Solution/Sample Diluent（20×）Concentrate，	50ml,20X concentrated
AOZ High Standard	AOZ spiking solution	1ml，50ng/ml
Substrate solution	Substrate Solution	14mL
Stop fluid	Stop Solution	8mL
Microplate Cover	Plate Cover