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Evergreen Sulfonamide Total Test Kit

The total amount of Evergreen sulfonamides detection kit used immunological principle to detect the residues of sulfonamides in honey in vitro. Sulfonamides are a class of organic synthetic compounds that are effective in treating bacterial and protozoal infections. Because these drugs can cause resistance in the body's immune system, they need to be monitored. Many countries have established maximum residue limits for sulfonamides, and the European Union, Jian'an University and the United States stipulate that the total amount of sulfonamides in edible tissues cannot exceed 100ppb. Detection principle The kit uses enzyme-linked immunosorbent assay to detect sulfonamide. The antigen is coated in a microplate, and the standard/sample and the antibody are added to the microplate sequentially. During incubation, the sulfonamide and the enzyme-labeled conjugate in the sample compete for binding to the sulfonamide antibody. After incubation, unbound enzyme-labeled conjugate and remaining antibody were removed by washing. The substrate reagent is then added, and the enzyme label reacts with the substrate. After incubation, stop solution was added to stop the reaction and the solution turned yellow. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. Kit composition reagent number Chinese name English name specification FT08 standard STANDARD ready-to-use type, 1ml/bottle FT08-008 microplate MICROTITERPLATE12 × 8 wells, coated antibody antigen FT08-009 washing buffer (50 ×)WASHBUFFER1 × 20ml, if the whole bottle is used, 20ml concentrate 980ml double deionized water, mix well. After dilution, it can be stored at 2-8 ℃ for 30 days. FT08-010 stop solution STOPSOLUTION1 mL × 8mL, colorless liquid, transparent bottle, red screw cap, dilute hydrochloric acid solution, ready-to-use FT08-011 substrate solution SUBSTRATESOLUTION1 mL × 14ml, transparent solution, can directly use FT08-012 enzyme marker CONJUGATE1 mL × 0.2mL, amber bottle FT08-014 sample diluent SAMPLEDILUENT1 mL × 30mL,20 × concentration, if dilute the whole bottle, 570ml double deionized water should be added and used on the same day. Microplate Cover PLATECOVER Incubation Cover Microplate Instructions IFU

Classification:

Evergreen Veterinary Drug Residues ELISA Kit

Key words:

Biotin

Product number:

BXEFT08A

Specifications:

96 well

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Product Description

EvergreenThe total amount of sulfonamide detection kit uses immunological principles to detect sulfonamide residues in honey in vitro.Sulfonamides are a class of organic synthetic compounds that are effective in treating bacterial and protozoal infections. Because these drugs can cause resistance in the body's immune system, they need to be monitored. Many countries have established maximum residue limits for sulfonamides, and the European Union, Jian'an University and the United States stipulate that the total amount of sulfonamides in edible tissues cannot exceed100ppb。

Detection principle

The kit uses enzyme-linked immunosorbent assay to detect sulfonamide. The antigen is coated in the microplate, and the standard is added to the microplate in turn./Samples and antibodies. During incubation, the sulfonamide and the enzyme-labeled conjugate in the sample compete for binding to the sulfonamide antibody. After incubation, unbound enzyme-labeled conjugate and remaining antibody were removed by washing. The substrate reagent is then added, and the enzyme label reacts with the substrate. After incubation, stop solution was added to stop the reaction and the solution turned yellow. The corresponding absorbance values were read using a microplate reader at a wavelength of 450 nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated.

Kit composition

Reagent No.	Chinese name	English Name	Specifications
FT08	Standard	STANDARD	ready-to-use, 1ml/bottle
FT08-008	Microplate	MICROTITER PLATE	12 x 8 wells, coated with antibody antigen
FT08-009	Wash buffer (50x)	WASH BUFFER	1 x 20ml, if using the whole bottle, 20ml concentrate 980ml double deionized water, mix well. After dilution, it can be stored at 2-8 ℃ for 30 days.
FT08-010	Stop fluid	STOP SOLUTION	1 × 8mL, colorless liquid, transparent bottle, red screw cap, dilute hydrochloric acid solution, ready-to-use
FT08-011	Substrate solution	SUBSTRATE SOLUTION	1 x 14ml, transparent solution, can be used directly
FT08-012	enzyme marker	CONJUGATE	1 x 0.2mL, amber bottle
FT08-014	Sample Diluent	SAMPLE DILUENT	1 × 30mL, 20 × concentrated. If the whole bottle is diluted, 570ml of double deionized water should be added and used on the same day.
	Microplate Cover	PLATE COVER	Cover microplate when incubating
	Instructions	IFU