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Evergreen Bovine Ig G ELISA Kit
IgG is the most important immunoglobulin in livestock and human serum and plays a major role in antibody defense mechanisms. The content of bovine IgG in serum is 17-27 mg/ml, but it will change with season. IgG is also the predominant immunoglobulin in the colostrum of livestock, including cattle. The content of IgG in bovine colostrum is 34-80 mg/ml, while the content of IgG in cow's milk is 0.5-7.5 mg/ml.
Classification:
Evergreen Protein Assay Kit
Key words:
Product number:
EC30611
Specifications:
96 times
Product Description
IgG is the most important immunoglobulin in livestock and human serum and plays a major role in antibody defense mechanisms. The content of bovine IgG in serum is 17-27 mg/ml, but it will change with season. IgG is also the predominant immunoglobulin in the colostrum of livestock, including cattle. The content of IgG in bovine colostrum is 34-80 mg/ml, while the content of IgG in cow's milk is 0.5-7.5 mg/ml.
Evergreen TREATMCowThe IgG ELISA kit is rapid, sensitive and reliable for quantitative detection of naturally active bovine IgG in milk and milk powder. The kit contains all the reagents required for the ELISA reaction. The kit is sufficient for 96 tests (including standards).
Detection principle
This kit is the use of double anti sandwich enzyme-linked immunosorbent assay method for detection. Microplate coated with anti-cattleIg G antibody, after adding bovine Ig G standard or sample, free bovine Ig G binds with the pre-coated anti-bovine Ig G antibody on the microplate, the captured bovine Ig G binds with the enzyme-labeled anti-bovine Ig G antibody again, develops color with TMB substrate, changes color from blue to yellow after adding acidic stop solution, and measures absorbance value with microplate reader at 450nm wavelength, the absorbance value is proportional to the content of bovine Ig G in the sample. The test results can be obtained by comparing the absorbance value of the sample with the absorbance value of the standard provided by the kit.
specificity
Ability to specifically detect cattleIgG does not react specifically with other bovine immunoglobulins or other bovine serum proteins.
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