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Evergreen Lactoperoxidase Activity Assay Kit
Lactoperoxidase (LPO) is a naturally occurring enzyme in milk. It is the most important peroxidase capable of oxidizing the thiocyanate ion (SCN-) in the presence of hydrogen peroxide (H2O 2). In milk, the main function of LPO is to catalyze the oxidation of molecules to produce products with a wide range of antibacterial activity. LPO plays an important role in the immune system of dairy cows. In the dairy industry, LPO is also an important intrinsic indicator for monitoring and assessing the severity of heat treatment, as it is one of the most thermostable enzymes in milk, which cannot be inactivated by an ordinary pasteurization process (63°C for 30 minutes or 72°C for 15 seconds), partially inactivated by an instantaneous pasteurization process at 74°C, and completely inactivated by UHT or ultra-high temperature pasteurization. Measuring LPO activity has been widely used to assess the extent and efficiency of mild heat treatment.
Classification:
Evergreen Lactoperoxidase Assay Kit
Key words:
Product number:
EC40111
Specifications:
96 times
Product Description
Lactoperoxidase (LPO) is a naturally occurring enzyme in milk. It is the most important peroxidase and can be used in hydrogen peroxide (H2O 2) in the presence of oxidized thiocyanate ions (SCN-). In milk, the main function of LPO is to catalyze the oxidation of molecules to produce products with a wide range of antibacterial activity. LPO plays an important role in the immune system of dairy cows.
In the dairy industry,LPO is also an important intrinsic indicator for monitoring and evaluating the severity of heat treatment, as it is one of the most thermostable enzymes in milk, the common pasteurization process(63°C for 30 minutes or 72°C for 15 seconds) cannot inactivate it,The instantaneous pasteurization process at 74 ℃ can partially inactivate it, and UHT or ultra-high temperature pasteurization can completely inactivate it. Measuring LPO activity has been widely used to assess the extent and efficiency of mild heat treatment.
The activity of LPO in pasteurized milk represents the quality of the product, since usually only raw milk containing high microbial quality can be processed by ordinary pasteurization. The quantification of lactate oxidase in pasteurized milk is related to the nutritional quality of milk. The high content of peroxidase indicates that the milk retains the original quality.
Evergreen TREATMThe Lactoperoxidase Activity Detection Kit is based on a colorimetric method for the rapid and reliable quantitative determination of lactoperoxidase activity in milk. The kit is sufficient96 tests (including standard).
Group StandardsT/CIFST-2022Colorimetric method for the determination of lactoperoxidase activity in milk
Product features:
✅Principle: Enzymatic reaction
✅Measurement wavelength:450nm
✅Sample handling:15-20Minutes
✅Detection time: about10Minutes
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