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Evergreen Ochratoxin A Test Kit
Ochratoxin A is a toxic metabolite produced by Aspergillus flavus and Penicillium (including Aspergillus ochratans). It has renal toxicity and carcinogenicity, and can lead to Balkan endemic nephropathy (BEN, chronic kidney disease related to renal system tumors), and can also lead to renal damage in pigs, and stunted growth of turkeys and chickens, reduced feed utilization, reduced egg production and eggshell quality, kidney disease and a large number of deaths (turkeys also have symptoms of refusal to eat). Most agricultural products contain ochratoxin A, which is mainly detected in grain products such as food and feed. Ochratoxin A is frequently detected in cereals and animal feed, in addition to fresh and roasted coffee, cocoa, spices and grape products such as raisins, grape juice and wine. The Evergreen Ochratoxin A detection kit can quantitatively detect Ochratoxin A in fresh coffee, roasted coffee, instant coffee, cocoa powder, cocoa butter, various spices and cereals. Detection principle Evergreen ochratoxin A detection kit uses direct solid phase enzyme-linked immunosorbent assay to detect ochratoxin A in samples. The microplate is coated with ochratoxin A antibody, the ochratoxin A in the standard or sample is combined with the antibody coated in the microhole, and then the ochratoxin A enzyme label added is combined with the remaining antibody. After incubation the contents of the wells were decanted and washed. Then the substrate reagent is added to react with the ochratoxin A enzyme label, and the solution turns blue. The color intensity is proportional to the concentration of the ochratoxin A enzyme label and inversely proportional to the concentration of ochratoxin A in the sample or standard. Finally the stop solution was added and the solution turned from blue to yellow. Use a microplate reader to detect the OD value of the solution in the micropore at 450nm, and compare the OD value of the sample with the OD value of the standard, and the result can be obtained. The kit consists of Chinese name, English name, specification, quantity, microplate AntibodycoatedmicrowellPlate12, 8 wells/strip, coated with ochratoxin A antibody 1 plate mixed well (red) Mixingwells12, 8 wells/strip, uncoated with antibody, red 1 plate ochratoxin A standard OchratoxinAStandards 1.5mL per bottle, black cap, 6 bottles can be directly used to detect diluent AssayDiluent12ml/bottle, brown cap 2 bottles of ochratoxin A enzyme label OchratoxinAHRP-conjugate12ml/bottle, green cap, can directly use 1 bottle of substrate reagent SubstrateReagent12mL, blue cap, stable tetramethylbenzidine (TMB), can directly use 1 bottle of stop solution StopSolution12mL, red cap, acidic solution, can directly use 1 bottle of washing buffer Washbuffer(PBS-T) PBS buffer containing 0.05 Tween20, diluted to 1L with distilled water before use, refrigerated 1 bag
Classification:
Evergreen Mycotoxin ELISA Kit
Key words:
Product number:
EB10712
Specifications:
96 well
Product Description
Ochratoxin Aare molds such as Aspergillus flavus and Penicillium(including Aspergillus ochraceus）Toxic metabolites produced, which are nephrotoxic and carcinogenic and can lead to Balkan endemic nephropathy (BEN, chronic kidney disease associated with tumors of the renal system), and can also lead to renal impairment in pigs, as well as stunted growth in turkeys and chickens, reduced feed utilization, reduced egg production and eggshell quality, kidney disease, and massive death (turkeys also have symptoms of refusal to eat).. Most agricultural products contain ochratoxin A, the current masterTo detect the content of food, feed and other grain products. Ochratoxin is frequently detected in cereals and animal feed A, in addition, fresh and roasted coffee, cocoa, spicesand grape products such as raisins, grape juice and wine also often contain ochratoxin A。
EvergreenOchratoxin AThe detection kit can quantitatively detect fresh coffee,Ochratoxin in roasted coffee, instant coffee, cocoa powder, cocoa butter, various spices and cereals A。
Detection principle
EvergreenOchratoxin AThe detection kit uses direct solid-phase enzyme-linked immunosorbent assay to detect ochratoxin in samples A. Microplate coated with ochratoxin AOchratoxin in antibodies, standards or samples ABinding to antibodies coated in microwells, followed by addition of ochratoxin Aenzyme label, which binds to the remaining antibody. After incubationThe contents of the wells were poured out and washed. Then add substrate reagent, with ochratoxin AThe enzyme label reacts, the solution turns blue, and the color intensity is the same as that of ochratoxin. AEnzymeThe concentration of the standard is proportional to the concentration of ochratoxin in the sample or standard. AThe concentration is inversely proportional. Finally the stop solution was added and the solution turned from blue to yellow. Use of microplate readerIn 450nmDetection of solutions in micropores under ODvalue, comparison sample ODValue and standard ODvalue, you can get the result.
Kit composition
|
Chinese name |
English Name |
Specifications |
Quantity |
|
Microplate |
Antibody coated microwell Plate |
12, 8 holes/strip, coated with ochratoxin A antibody |
1 Plate |
|
Mixing hole (red) |
Mixing wells |
12 bars, 8 wells/bar, uncoated with antibody, red |
1 Plate |
|
Ochratoxin A Standard |
Ochratoxin A Standards |
1.5mL per bottle, black cap, can be used directly |
6 bottles |
|
Detection of diluent |
Assay Diluent |
12ml/bottle, brown cap |
2 bottles |
|
Ochratoxin A Enzyme Label |
Ochratoxin A HRP-conjugate |
12ml/bottle, green cap, can be used directly |
1 bottle |
|
substrate reagent |
Substrate Reagent |
12mL, blue cover, stable tetramethylbenzidine (TMB), can be used directly |
1 bottle |
|
Stop fluid |
Stop Solution |
12mL, red cover, acid solution, can be used directly |
1 bottle |
|
Wash buffer |
Wash buffer (PBS-T) |
PBS buffer containing 0.05%Tween 20, diluted with distilled water 1L, refrigerated |
1 bag |
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