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Zhongjian Baotai

Beijing Biotai Co., Ltd. and all staff are dedicate-d to providing customers with the best techni-cal solutions, quality products and after-sales services, and strive to promote the developme-nt of China's food safety industry.

TEL:400-1888-518

Mycotoxin Tests

Evergreen Fumisin Assay Kit

Fumonisin (B1, B2, B3) is a group of mycotoxins produced by Fusarium moniliforme. It widely exists in nature, mainly contaminates corn, and can cause liver cancer in rats, pulmonary edema in pigs and leukomalacia in horses. In South Africa and China, areas with high levels of fumonisins in maize are areas of high incidence of esophageal cancer. Evergreen fumatoxin detection kit can quantitatively detect the content of fumatoxin in grain and feed. Detection principle Evergreen fumosin detection kit uses solid-phase direct competitive enzyme-linked immunosorbent principle to detect fumosin in samples. Microwells are coated with a fumonisin-specific monoclonal antibody that cross-reacts with three subtypes of fumonisin. The fumonisin in the standard/sample competes with the fumonisin enzyme label for binding to the binding site on the antibody. After completion of the reaction, the plate was washed to remove non-specific reactants. Substrate reagent (TMB) is added to the microwell, and the solution turns blue, with color intensity proportional to the concentration of the fumarotoxin enzyme label and inversely proportional to the concentration of the fumarotoxin in the sample or standard. The stop solution was then added and the color changed from blue to yellow. Use a microplate reader to detect the OD value of the solution in the micropore at 450nm, and compare the OD value of the sample with the OD value of the standard, and the result can be obtained. The kit consists of Chinese name, English name, specification and quantity of micropores. The Antibodycoatedmicrowells are placed in a microplate, with a total of 96 micropores (12, 8 wells/strip). One bag of dilution wells(green) coated with mouse anti-fumonisin monoclonal antibody is Dilutionwells(green) and placed in a microplate, with a total of 96 micropores, uncoated antibody (12 bars, 8 holes/bar) 1 plate of fumaritoxin standard FumonisinStandards fumaritoxin aqueous solution, 1.5mL6 bottles of fumaritoxin enzyme standard per bottle FumonisinHRPconjugate the conjugate of fumaritoxin and peroxidase, fumaritoxin enzyme standard dissolved in preservative-containing buffer solution, 12mL2 bottles of substrate reagent SubstrateReagent15mL per bottle, 1 bottle of stable tetramethylbenzidine (TMB) termination liquid StopSolution15mL, acid solution 1 bottle of washing buffer Washingbuffer PBS buffer containing 0.05% Tween 20, diluted to 1L with distilled water before use, refrigerated 1 bag

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Evergreen Ochratoxin A Test Kit

Ochratoxin A is a toxic metabolite produced by Aspergillus flavus and Penicillium (including Aspergillus ochratans). It has renal toxicity and carcinogenicity, and can lead to Balkan endemic nephropathy (BEN, chronic kidney disease related to renal system tumors), and can also lead to renal damage in pigs, and stunted growth of turkeys and chickens, reduced feed utilization, reduced egg production and eggshell quality, kidney disease and a large number of deaths (turkeys also have symptoms of refusal to eat). Most agricultural products contain ochratoxin A, which is mainly detected in grain products such as food and feed. Ochratoxin A is frequently detected in cereals and animal feed, in addition to fresh and roasted coffee, cocoa, spices and grape products such as raisins, grape juice and wine. The Evergreen Ochratoxin A detection kit can quantitatively detect Ochratoxin A in fresh coffee, roasted coffee, instant coffee, cocoa powder, cocoa butter, various spices and cereals. Detection principle Evergreen ochratoxin A detection kit uses direct solid phase enzyme-linked immunosorbent assay to detect ochratoxin A in samples. The microplate is coated with ochratoxin A antibody, the ochratoxin A in the standard or sample is combined with the antibody coated in the microhole, and then the ochratoxin A enzyme label added is combined with the remaining antibody. After incubation the contents of the wells were decanted and washed. Then the substrate reagent is added to react with the ochratoxin A enzyme label, and the solution turns blue. The color intensity is proportional to the concentration of the ochratoxin A enzyme label and inversely proportional to the concentration of ochratoxin A in the sample or standard. Finally the stop solution was added and the solution turned from blue to yellow. Use a microplate reader to detect the OD value of the solution in the micropore at 450nm, and compare the OD value of the sample with the OD value of the standard, and the result can be obtained. The kit consists of Chinese name, English name, specification, quantity, microplate AntibodycoatedmicrowellPlate12, 8 wells/strip, coated with ochratoxin A antibody 1 plate mixed well (red) Mixingwells12, 8 wells/strip, uncoated with antibody, red 1 plate ochratoxin A standard OchratoxinAStandards 1.5mL per bottle, black cap, 6 bottles can be directly used to detect diluent AssayDiluent12ml/bottle, brown cap 2 bottles of ochratoxin A enzyme label OchratoxinAHRP-conjugate12ml/bottle, green cap, can directly use 1 bottle of substrate reagent SubstrateReagent12mL, blue cap, stable tetramethylbenzidine (TMB), can directly use 1 bottle of stop solution StopSolution12mL, red cap, acidic solution, can directly use 1 bottle of washing buffer Washbuffer(PBS-T) PBS buffer containing 0.05 Tween20, diluted to 1L with distilled water before use, refrigerated 1 bag

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Evergreen vomiting toxin rapid test kit

Deoxynivalenol (DON) belongs to the trichothecene group of compounds, mainly produced by Fusarium graminearum, Fusarium oxysporum, Fusarium moniliforme, Fusarium moniliforme, Fusarium pink, and Fusarium moniliforme. It is commonly found in plant products, particularly in cereals. Vomitoxin concentrations in wheat, corn and rice can reach ppm levels. Due to its high cytotoxicity and immunosuppressive properties, it poses a threat to the health of humans and animals. EvergreenTREATM Vomitoxin detection kit provides a rapid, sensitive and reliable quantitative analysis method. The kit contains all the reagents needed for enzyme-linked immunoassay. The kit can carry out 96 tests (including standard substances). The detection principle of the EvergreenTREATM vomiting toxin detection kit is a competitive enzyme-linked immunoassay (EISA). Microwells are coated with vomitoxin antigen, and vomitoxin standard/sample and vomitoxin antibody are added to the microwells. Free vomitoxin and vomitoxin antigen coated in the microwells compete for binding sites on the vomitoxin antibody. Unbound vomitoxin and vomitoxin antibody are removed during the first wash. Horseradish peroxidase-labeled secondary antibody (HRP) is then added and unbound enzyme-labeled conjugate is removed in a second washing step. Substrate reagent is then added to each well and the bound enzyme conjugate catalyzes the substrate solution to turn blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of vomitoxin in the sample or standard. The kit consists of Chinese name, English name, specification, quantity, microplate Microtiterplate, 96 micropores (12, 8 wells/strip), coated with DON-OVA1 bags of standard solution Standardssolutions 1mL per bottle, ready-to-use 6 bottles of sample diluent SampleDilutent15ml, white cap, ready-to-use 1 bottle of antibody solution Anti-DONantibody6ml, white cap, ready-to-use type. 1 bottle of substrate TMBSubstrate12mL, brown cap, stable tetramethylbenzidine (TMB), I .e., 1 bottle of enzyme-labeled conjugate HRPConjugate12ml, green cap, I .e., 1 bottle of termination solution StopSolution6mL, red cap, containing 0.18M sulfuric acid, I .e., 1 bottle of washing solution is concentrated WashSolution60ml20 times, and 1:20 dilution (e.g., 1ml20 × washing solution 19ml distilled water) is required for use

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Evergreen Zearalenone Rapid Test Kit

Zearalenone (ZEA) is a metabolite of Fusarium and Gibberella spp. Zearalenone contamination is very common, like corn, wheat, oats, barley and sorghum are very easy to contaminate zearalenone. Ingestion of zearalenone by animals, particularly pigs, can lead to infertility, miscarriage or other reproductive problems. TREATM zearalenone detection kit can quantitatively determine zearalenone in corn and wheat samples, which is rapid, sensitive and reliable. The kit contains all the reagents required for the enzyme-linked immunoassay, and the kit can perform 96 tests (including standards). Detection principle EvergreenTREATM zearalenone detection kit is a competitive enzyme-linked immunoassay (EISA). Zearalenone standard/sample and zearalenone antibody are added to the microwells coated with zearalenone antigen, free zearalenone and zearalenone antigen coated in the microwells compete for binding sites on the zearalenone antibody, and unbound zearalenone and zearalenone antibodies are removed during the first wash. Horseradish peroxidase-labeled secondary antibody (HRP) is then added and unbound enzyme-labeled conjugate is removed in a second washing step. Substrate reagent is then added to each well and the bound enzyme conjugate catalyzes the substrate solution to turn blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of zearalenone in the sample or standard. The kit consists of Chinese name, English name, specification, quantity, microplate Microtiterplate, a total of 96 micropores (12, 8 wells/strip), coated with ZEA-OVA1 bags of standard solution Standardssolutions0ppb,2ml2ppb,5ppb,10ppb,25ppb,100ppb, 1mL per bottle, ready-to-use. 6 bottles of sample diluent SampleDiluent15ml, white cap, ready-to-use type 1 bottle of antibody solution Anti-ZEA antibody6ml, white cap, ready-to-use type. 1 bottle of substrate TMBSubstrate12mL, brown cap, stable tetramethylbenzidine (TMB), ready-to-use 1 bottle of enzyme-labeled conjugate HRPConjugate6ml, green cap, ready-to-use 1 bottle of stop solution StopSolution6mL, red cap, containing 0.18M sulfuric acid, ready-to-use 1 bottle of washing solution WashSolution60ml,20 times concentrated 1 bottle

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Evergreen Aflatoxin B1 Highly Sensitive Kit-Milk & Dairy Products

Aflatoxins are toxic metabolites produced by a variety of molds (such as Aspergillus flavus and Aspergillus parasiticus) and are highly carcinogenic. Aflatoxin B1 is usually present in cereals, cottonseeds and nuts at the same time as aflatoxins B2, G1, G2. EvergreenTREATM Aflatoxin B1 high sensitive detection kit was used to quantitatively detect aflatoxin B1 in milk and milk by competitive enzyme-linked immunosorbent assay. The kit contains all reagents required for the reaction-including standards, enough for 96 assays (including standards). Quantitative detection requires a microplate reader. Detection principle Aflatoxin B1 test kit is based on antigen-antibody reaction. The microwells are coated with an antigen that can bind to the aflatoxin B1 antibody. Standard or sample and enzyme labeled antibody are added to the microwells. Free aflatoxin and antigen coated in the microwell compete for binding to the binding site of the enzyme-labeled antibody. Unbound enzyme-labeled antibody is removed in a washing step. Substrate reagent was then added and the solution turned blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of aflatoxin B1 in the sample or standard. The kit consists of Chinese name, English name, specification, quantity, microplate MicroteterPlate, 96 micropores (12, 8 wells/strip), 1 bag of standard solution Standards solutions coated with antigen, 1mL per bottle, free aflatoxin B1, ready-to-use 6 bottles of sample diluent SampleDiluent15ml, white cap, ready-to-use 1 bottle of substrate TMBSubstrate12 mL, brown cap, stable tetramethylbenzidine (TMB), the ready-to-use 1 bottle of enzyme-labeled conjugate HRPConjugate6ml, green cap, ready-to-use 1 bottle of stop solution StopSolution6 mL, red cap, containing 0.18M sulfuric acid, ready-to-use 1 bottle of washing solution WashSolution60ml,20 times concentrated 1 bottle

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Evergreen Aflatoxin B1 High Sensitive Kit-Cereal and Soya-based Formula

Aflatoxins are toxic metabolites produced by a variety of molds (such as Aspergillus flavus and Aspergillus parasiticus) and are highly carcinogenic. Aflatoxin B1 is usually present in cereals, cottonseeds and nuts at the same time as aflatoxins B2, G1, G2. The EvergreenTREATM aflatoxin B1 highly sensitive detection kit uses the principle of competitive enzyme-linked immunosorbent assay to quantitatively detect aflatoxin B1 in cereal and soy-based formula foods. The kit contains all reagents required for the reaction-including standards, enough for 96 assays (including standards). Quantitative detection requires a microplate reader. Detection principle Aflatoxin B1 test kit is based on antigen-antibody reaction. The microwells are coated with an antigen that can bind to the aflatoxin B1 antibody. Standard or sample and enzyme labeled antibody are added to the microwells. Free aflatoxin and antigen coated in the microwell compete for binding to the binding site of the enzyme-labeled antibody. Unbound enzyme-labeled antibody is removed in a washing step. Substrate reagent was then added and the solution turned blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of aflatoxin B1 in the sample or standard. The kit consists of Chinese name, English name, specification, quantity, microplate MicroteterPlate, 96 micropores (12, 8 wells/strip), 1 bag of standard solution Standards solutions coated with antigen, 1mL per bottle, free aflatoxin B1, ready-to-use 6 bottles of sample diluent SampleDiluent15ml, white cap, ready-to-use 1 bottle of substrate TMBSubstrate12 mL, brown cap, stable tetramethylbenzidine (TMB), the ready-to-use 1 bottle of enzyme-labeled conjugate HRPConjugate6ml, green cap, ready-to-use 1 bottle of stop solution StopSolution6 mL, red cap, containing 0.18M sulfuric acid, ready-to-use 1 bottle of washing solution WashSolution60ml20 times concentrated 1 bottle

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Evergreen Aflatoxin M1 Rapid Test Kit

Aflatoxins are toxic secondary metabolites produced by a variety of molds (such as Aspergillus flavus and Aspergillus parasiticus) and are highly carcinogenic. Aflatoxins are commonly found in grains, nuts, and other crop products. Aflatoxins are potential carcinogens and immunosuppressants. Aflatoxins have different subtypes, with B1 being the most common and the most toxic. When cows ingest contaminated feed, aflatoxin B1 occurs hydroxylation reaction to produce aflatoxin M1, aflatoxin M1 can enter the milk with lactation. Aflatoxin M1 is stable throughout the bus disinfection process and can enter the final product for human consumption. Most countries around the world have established limits for aflatoxin M1 in milk and dairy products. The US and EU set a limit of 50ppt for aflatoxin M1 in milk and reconstituted milk powder. The TREA Aflatoxin M1 Rapid Test Kit can detect aflatoxin M1 in milk and dairy products qualitatively or quantitatively. The kit contains all reagents required for ELISA experiments, including standards. The kit can perform 96 tests. Scope of application Evergreen aflatoxin M1 rapid test kit is suitable for qualitative or quantitative detection of aflatoxin M1 in milk or milk products. 2. Detection principle TREA aflatoxin M1 detection kit is based on antigen-antibody reaction. The microwells were coated with aflatoxin M1 antibody. The aflatoxin standard or sample and the enzyme label conjugate are added to the microwells. Free aflatoxin M1 and the enzyme-labeled conjugate compete for binding to the antibody in the microwell. Unbound enzyme-labeled conjugate is removed in a washing step. Substrate reagent was then added and the solution turned blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of aflatoxin M1 in the sample or standard. 3. Kit Composition Enough reagents for 96 reactions were included in the kit. Chinese name English name specification quantity microplate AflatoxinM1plate a total of 96 microholes (12, 8 wells/strip), coated with aflatoxin M1 antibody 1 bag of standard solution Standards solutions 1mL per bottle, ready-to-use 6 bottles of substrate TMBSubstrate12mL, brown cap, stable tetramethylbenzidine (TMB), ready-to-use 1 bottle of enzyme-labeled HRPConjugate6mL, green cap, horseradish peroxidase labeled aflatoxin, one bottle of ready-to-use stop solution StopSolution6 mL, red cap, containing 0.18M sulfuric acid, one bottle of ready-to-use washing solution WashSolution60ml20-fold concentrated, one bottle of sample diluent SampleDiluent50mL, one bottle of ready-to-use

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