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Beijing Biotai Co., Ltd. and all staff are dedicate-d to providing customers with the best techni-cal solutions, quality products and after-sales services, and strive to promote the developme-nt of China's food safety industry.

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Evergreen Chloramphenicol Test Kit-Highly Sensitive

Chloramphenicol is associated with aplastic anemia in humans, can affect reproduction in animals, and is hepatotoxic. Chloramphenicol is a broad-spectrum antibiotic widely used in livestock and poultry breeding, which can kill the main gram-negative bacteria in animals. Many countries prohibit the use of chloramphenicol in food animals. Therefore, it is necessary to monitor chloramphenicol in foods such as meat, milk, honey and aquatic products. Biorex Chloramphenicol Detection Kit-highly sensitive detection of chloramphenicol in honey, muscle, eggs and seafood/shrimp using the principle of enzyme-linked immunosorbent assay. Detection principle The kit uses enzyme-linked immunosorbent assay to detect chloramphenicol. The micropores on the enzyme label plate provided by the kit are pre-coated with secondary antibodies. Standards or samples, enzyme labels and antibodies are added to the microwells. During incubation, the chloramphenicol in the standard/sample simultaneously competes with the enzyme label for the binding site on the antibody. Unbound enzyme-labeled conjugate is removed by washing. Then the substrate reagent is added, and the enzyme label reacts with the substrate. After incubation, the stop solution is added to terminate the reaction. The corresponding absorbance values were read using a microplate reader at a wavelength of 450nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated. The kit consists of Chinese name, English name, specification, microplate Microtitreplate12 × 8 wells, coated with secondary chloramphenicol standard Standards6 bottle, 1.5ml/bottle enzyme marker EnzymeConjugate 250μL/bottle, amber bottle enzyme marker diluent EnzymeConjugateDiluent1 × 12ml, red solution, plastic bottle antibody solution AntibodySolution1 × 12ml, blue solution, plastic bottle washing buffer (50 ×)WashSolution(50X)Concentrate1 × 20ml termination solution StopSolution1 × 9mL, red cap plastic bottle substrate solution SubstrateSolution1 ml × 24ml, amber plastic bottle sample diluent (5X)SampleDiluent5X2 ml × 25ml, blue cap plastic bottle high standard solution SpikingSolution1 ml × 1ml,100ng/ml microplate cap PLATECOVER cover microplate when incubated

Classification:

Evergreen Veterinary Drug Residues ELISA Kit

Key words:

Biotin

Product number:

BXEFB06A

Specifications:

96 well

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Product Description

EvergreenChloramphenicol detection kit-highly sensitive detection of chloramphenicol in honey, muscle, eggs and seafood/shrimp using enzyme-linked immunosorbent principle.

Detection principle

The kit used enzyme-linked immunosorbent assay to detect chloramphenicol. The micropores on the enzyme label plate provided by the kit are pre-coated with secondary antibodies. Standards or samples, enzyme labels and antibodies are added to the microwells. During incubation, the standardThe chloramphenicol in the sample competes with the enzyme label simultaneously for the binding site on the antibody. Unbound enzyme-labeled conjugate is removed by washing. Then the substrate reagent is added, and the enzyme label reacts with the substrate. After incubation, the stop solution is added to terminate the reaction. The corresponding absorbance values were read using a microplate reader at a wavelength of 450 nm. The depth of color is inversely proportional to the content in the standard/sample, a standard curve is constructed, and the concentration in the sample is calculated.

Kit composition

Chinese name	English Name	Specifications
Microplate	Flat Microtitre	12 × 8 wells, coated with secondary antibody
Chloramphenicol Standard	Standards	6 bottles, 1.5ml/bottle
enzyme marker	Enzyme Conjugate	250 μL/bottle, amber bottle
enzyme marker dilution	Enzyme Conjugate Diluent	1 x 12ml, red solution, plastic bottle
Antibody solution	Antibody Solution	1 x 12ml, blue solution, plastic bottle
Wash buffer (50x)	Wash Solution (50X) Concentrate	1 × 20ml
Stop fluid	Stop Solution	1 × 9mL, red cap plastic bottle
Substrate solution	Substrate Solution	1 x 24ml, amber plastic bottle
Sample Diluent (5X)	Sample Diluent 5X	2 x 25ml, blue cap plastic bottle
high standard solution	Spiking Solution	1 × 1ml ,100ng/ml
Microplate Cover	PLATE COVER	Cover microplate when incubating