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Evergreen vomiting toxin rapid test kit
Deoxynivalenol (DON) belongs to the trichothecene group of compounds, mainly produced by Fusarium graminearum, Fusarium oxysporum, Fusarium moniliforme, Fusarium moniliforme, Fusarium pink, and Fusarium moniliforme. It is commonly found in plant products, particularly in cereals. Vomitoxin concentrations in wheat, corn and rice can reach ppm levels. Due to its high cytotoxicity and immunosuppressive properties, it poses a threat to the health of humans and animals. EvergreenTREATM Vomitoxin detection kit provides a rapid, sensitive and reliable quantitative analysis method. The kit contains all the reagents needed for enzyme-linked immunoassay. The kit can carry out 96 tests (including standard substances). The detection principle of the EvergreenTREATM vomiting toxin detection kit is a competitive enzyme-linked immunoassay (EISA). Microwells are coated with vomitoxin antigen, and vomitoxin standard/sample and vomitoxin antibody are added to the microwells. Free vomitoxin and vomitoxin antigen coated in the microwells compete for binding sites on the vomitoxin antibody. Unbound vomitoxin and vomitoxin antibody are removed during the first wash. Horseradish peroxidase-labeled secondary antibody (HRP) is then added and unbound enzyme-labeled conjugate is removed in a second washing step. Substrate reagent is then added to each well and the bound enzyme conjugate catalyzes the substrate solution to turn blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of vomitoxin in the sample or standard. The kit consists of Chinese name, English name, specification, quantity, microplate Microtiterplate, 96 micropores (12, 8 wells/strip), coated with DON-OVA1 bags of standard solution Standardssolutions 1mL per bottle, ready-to-use 6 bottles of sample diluent SampleDilutent15ml, white cap, ready-to-use 1 bottle of antibody solution Anti-DONantibody6ml, white cap, ready-to-use type. 1 bottle of substrate TMBSubstrate12mL, brown cap, stable tetramethylbenzidine (TMB), I .e., 1 bottle of enzyme-labeled conjugate HRPConjugate12ml, green cap, I .e., 1 bottle of termination solution StopSolution6mL, red cap, containing 0.18M sulfuric acid, I .e., 1 bottle of washing solution is concentrated WashSolution60ml20 times, and 1:20 dilution (e.g., 1ml20 × washing solution 19ml distilled water) is required for use
Classification:
Evergreen Mycotoxin ELISA Kit
Key words:
Product number:
EB10510R
Specifications:
96 well
Product Description
Deoxynivalenol (DON)It belongs to the trichothecene family of compounds, mainly produced by Fusarium graminearum, Fusarium oxysporum, Fusarium moniliforme, Fusarium pseudocladosporium, Fusarium pink, and Fusarium oxysporum. It is commonly found in plant products, particularly in cereals. wheat,
The concentration of vomitoxin in corn and rice can reach ppm level. Due to its high cytotoxicity and immunosuppressive properties, it poses a threat to the health of humans and animals.
Evergreen TREATMVomitoxin detection kit provides a rapid, sensitive and reliable quantitative analysis method. The kit contains all the reagents needed for the enzyme-linked immunoassay, and the kit can perform 96 tests (including standards).
Detection principle
Evergreen TREATMVomitoxin detection kit is a competitive enzyme-linked immunoassay (EISA). Microwells are coated with vomitoxin antigen, and vomitoxin standard/sample and vomitoxin antibody are added to the microwells. Free vomitoxin and vomitoxin antigen coated in the microwells compete for binding sites on the vomitoxin antibody. Unbound vomitoxin and vomitoxin antibody are removed during the first wash. Horseradish peroxidase-labeled secondary antibody (HRP) is then added and unbound enzyme-labeled conjugate is removed in a second washing step. Substrate reagent is then added to each well and the bound enzyme conjugate catalyzes the substrate solution to turn blue. Finally a stop solution was added to stop the reaction and turn the color of the solution in the microwells to yellow. Use a microplate reader to detect the OD value of the solution in the microwell at 450nm, and the absorbance value is inversely proportional to the concentration of vomitoxin in the sample or standard.
Kit composition
|
Chinese name |
English Name |
Specifications |
Quantity |
|
Microplate |
Microtiter plate |
A total of 96 micropores (12, 8 wells/strip) were coated with DON-OVA |
1 bag |
|
Standard solution |
Standards solutions |
1mL per bottle, ready to use |
6 bottles |
|
Sample Diluent |
Sample Dilutent |
15ml, white cover, ready-to-use type |
1 bottle |
|
Antibody solution |
Anti-DON antibody |
6ml, white cover, ready to use. |
1 bottle |
|
Substrate |
TMB Substrate |
12 mL, brown cap, stabilized tetramethylbenzidine (TMB), ready-to-use |
1 bottle |
|
Enzyme-labeled conjugates |
HRP Conjugate |
12ml, green cover, ready-to-use |
1 bottle |
|
Stop fluid |
Stop Solution |
6 mL, red cover, containing 0.18M sulfuric acid, ready-to-use |
1 bottle |
|
Wash solution |
Wash Solution |
60ml 20 times concentrated, 1:20 dilution is required when using (such as 1ml 20 x washing solution 19ml distilled water) |
1 bottle |
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